Recycle process for the production of low-cost soluble collagen
First Claim
1. A method for producing an aqueous solution of solubilized collagen comprising:
- a. providing an aqueous ground slurry of insoluble collagen and adjusting the pH of said slurry to obtain activity for a proteolytic enzyme added in Step b;
b. adding said proteolytic enzyme to said pH adjusted slurry;
c. reacting said slurry and enzyme of Step b and\or recycled insoluble collagen and enzyme of Step e at a temperature, T, and for a time, t, effective for forming a solution increased in solubilized collagen;
d. adding insoluble collagen with or without additional water to said solution of Step c and mixing;
e. separating at least some of the solution of Step d containing solubilized collagen from said insoluble collagen, whereby at least a portion of said insoluble collagen and proteolytic enzyme is recycled to Step c, and a separated solution containing solubilized collagen is withdrawn as product.
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Abstract
The invention includes a method that provides a low cost aqueous solution of solubilized collagen by the steps of: (a) providing an aqueous ground slurry of insoluble collagen and adjusting the pH of said slurry to obtain activity for a proteolytic enzyme added in Step b; (b) adding said proteolytic enzyme to said pH adjusted slurry; (c) reacting the slurry and enzyme of Step b and/or recycled insoluble collagen and enzyme from Step e at a temperature, T, and for a time, t, effective for forming a solution increased in solubilized collagen; (d) adding additional water and insoluble collagen to said solution of Step c and mixing; (e) separating at least some of the solution of Step d containing solubilized collagen from insoluble collagen, whereby at least a portion of said insoluble collagen and proteolytic enzyme is recycled to Step c, and the separated solution containing solubilized collagen is withdrawn as product; an alternative embodiment provides for the direct production of solubilized collagen without the recycle step.
84 Citations
52 Claims
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1. A method for producing an aqueous solution of solubilized collagen comprising:
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a. providing an aqueous ground slurry of insoluble collagen and adjusting the pH of said slurry to obtain activity for a proteolytic enzyme added in Step b; b. adding said proteolytic enzyme to said pH adjusted slurry; c. reacting said slurry and enzyme of Step b and\or recycled insoluble collagen and enzyme of Step e at a temperature, T, and for a time, t, effective for forming a solution increased in solubilized collagen; d. adding insoluble collagen with or without additional water to said solution of Step c and mixing; e. separating at least some of the solution of Step d containing solubilized collagen from said insoluble collagen, whereby at least a portion of said insoluble collagen and proteolytic enzyme is recycled to Step c, and a separated solution containing solubilized collagen is withdrawn as product. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 21)
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17. A method for producing an aqueous solution of solubilized collagen comprising:
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a. providing an aqueous ground slurry of insoluble collagen; b. adjusting the water or solid content of said wet ground slurry whereby said insoluble collagen is at a concentration that promotes substantially maximum solubilized collagen concentration and molecular weight in a final product; c. adjusting the pH of said slurry from Step b to obtain activity for a proteolytic enzyme added in Step d; d. adding and mixing said proteolytic enzyme with said pH adjusted slurry; e. reacting the slurry of Step d and\or said recycled insoluble collagen of Step g at a temperature, T, and for a time, t, effective for forming a solution comprising solubilized collagen derived from said insoluble collagen particles; f. adding insoluble collagen with or without additional water to said solution containing solubilized collagen in Step e and mixing; g. separating at least some of said solution of Step f containing solubilized collagen from said insoluble collagen and returning said insoluble collagen to Step e, whereby at least a portion of said proteolytic enzyme is recycled, and a separated solution containing solubilized collagen is withdrawn as product. - View Dependent Claims (18, 19, 20, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A method for producing a solution of soluble collagen, said method comprising the steps of:
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a. reacting insoluble collagen with a proteolytic enzyme to produce a solution including soluble collagen; b. separating at least some of the soluble collagen from the insoluble collagen; c. withdrawing the soluble collagen as product; and d. returning the separated insoluble collagen to Step a, whereby at least a portion of the proteolytic enzyme is recycled. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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Specification