Methods and reagents for lengthening telomeres

• US 5,686,306 A
• Filed: 11/10/1994
• Issued: 11/11/1997
• Est. Priority Date: 05/13/1992
• Status: Expired due to Term

• Current Assignee: Geron Corporation, Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: West, Michael D., Shay, Jerry, Wright, Woodring E.
• Primary Examiner(s): LeGuyader, John L.
• Assistant Examiner(s): LARSON, THOMAS GEORGE
• Application Number:
  US08/337,684
• Time in Patent Office: 1,097 Days
• Field of Search: 435/6, 435/70.2, 435/172.2, 435/91.1, 435/240.1, 435/240.2, 435/240.26, 435/325, 435/346, 435/375, 435/377, 435/384, 435/405, 514/44, 536/23.1, 536/24.1, 536/24.3, 536/24.33, 536/24.5
• US Class Current: 435/346
• CPC Class Codes: C12N 2501/70 Enzymes
• CPC Class Codes: C12N 5/0018 Culture media for cell or tissue culture media for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/163 one of the fusion partners being a B or a T lymphocyte
• CPC Class Codes: C12Y 207/07049 RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Abstract

Method and compositions for increasing telomere length in normal cells can be used to increase the proliferative capacity of cells and to delay the onset of cellular senescence.

Claims

1. A method for increasing the proliferative capacity of normal cells having telomerase activity, which method comprises culturing or cultivating said cells in the presence of an oligonucleotide substrate for telomerase under conditions such that said oligonucleotide substrate enters said cells and acts to lengthen telomeric DNA of said cells and the proliferative capacity of said cells is increased.

2. The method of claim 1, wherein said oligonucleotide substrate for telomerase consists of an oligonucleotide sequence selected from the group consisting of 5'"'"'-TTAGGG-3'"'"' (SEQ ID NO 2), 5'"'"'-TTAGGGTTA-3'"'"'(SEQ ID NO 3), 5'"'"'-TTAGGGTTAGGG-3'"'"'(SEQ ID NO 1), 5'"'"'-TCGAGCACAGTT-3'"'"'(SEQ ID NO 4), and 5'"'"'-(GXGXGX)₂ -3'"'"' (SEQ ID NO 5), in which X can be independently selected at each position from either T or A.

3. A method of making human hybridoma cells with increased proliferative capacity, said method comprising:

(a) culturing an immortal cell line in the presence of an oligonucleotide substrate for telomerase under conditions such that the telomeres in cells of said cell line lengthen, anti the proliferative capacity of said cells is increased; and

(b) fusing said cells cultured in step (a) with human antibody-producing cells to produce said hybridoma cells.