Enzyme capable of degrading cellulose or hemicellulose
First Claim
1. An isolated and purified DNA sequence which codes for a modified cellulose- or hemicellulose-degrading enzyme, wherein the enzyme comprises a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, and wherein the carbohydrate binding domain has the following core sequence:
- ##STR11## wherein;
Xaa in position 1 is Trp or Tyr;
Xaa in position 2 is Gly or Ala;
Xaa in position 7 is Gln, Ile or Asn;
Xaa in position 8 is Gly or Asn;
Xaa in position 9 is Trp, Phe or Tyr;
Xaa in position 10 is Ser, Asn, Thr or Gln;
Xaa in position 12 is Pro, Ala or Cys;
Xaa in position 13 is Thr, Arg or Lys;
Xaa in position 14 is Thr, Cys or Asn;
Xaa in position 16 is one of the twenty standard L-α
-amino acids found in proteins;
Xaa in position 17 is one of the twenty standard L-α
-amino acids found in proteins;
Xaa in position 18 is Gly or Pro;
Xaa in position 19 is Ser, Thr, Phe, Leu or Ala, or is absent;
Xaa in position 20 is Thr or Lys;
Xaa in position 22 is Val, Thr, Arg, Glu or Lys;
Xaa in position 23 is Lys, Gln or Ala;
Xaa in position 24 is Gln or Ile;
Xaa in position 26 is Gln, Asp or Ala;
Xaa in position 27 is Trp, Phe or Tyr;
Xaa in position 29 is Tyr, Ser, His or Ala;
Xaa in position 32 is Leu, Ile, Gln, Val or Thr; and
Xaa in position 33 is one of the twenty standard L-α
-amino acids found in proteins, or is absent.
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Accused Products
Abstract
The present invention relates to cellulose- or hemicellulose-degrading enzymes which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises the following amino acid sequence. ##STR1## or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.
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Citations
86 Claims
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1. An isolated and purified DNA sequence which codes for a modified cellulose- or hemicellulose-degrading enzyme, wherein the enzyme comprises a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, and wherein the carbohydrate binding domain has the following core sequence:
- ##STR11## wherein;
Xaa in position 1 is Trp or Tyr;Xaa in position 2 is Gly or Ala; Xaa in position 7 is Gln, Ile or Asn; Xaa in position 8 is Gly or Asn; Xaa in position 9 is Trp, Phe or Tyr; Xaa in position 10 is Ser, Asn, Thr or Gln; Xaa in position 12 is Pro, Ala or Cys; Xaa in position 13 is Thr, Arg or Lys; Xaa in position 14 is Thr, Cys or Asn; Xaa in position 16 is one of the twenty standard L-α
-amino acids found in proteins;Xaa in position 17 is one of the twenty standard L-α
-amino acids found in proteins;Xaa in position 18 is Gly or Pro; Xaa in position 19 is Ser, Thr, Phe, Leu or Ala, or is absent; Xaa in position 20 is Thr or Lys; Xaa in position 22 is Val, Thr, Arg, Glu or Lys; Xaa in position 23 is Lys, Gln or Ala; Xaa in position 24 is Gln or Ile; Xaa in position 26 is Gln, Asp or Ala; Xaa in position 27 is Trp, Phe or Tyr; Xaa in position 29 is Tyr, Ser, His or Ala; Xaa in position 32 is Leu, Ile, Gln, Val or Thr; and Xaa in position 33 is one of the twenty standard L-α
-amino acids found in proteins, or is absent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- ##STR11## wherein;
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24. An isolated and purified DNA sequence coding for a modified cellulose- or hemicellulose-degrading enzyme, wherein the enzyme comprises a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, and wherein the linking B region has an amino acid sequence selected from the group consisting of:
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Ala Arg Thr Asn Val Gly Gly Gly Ser Thr Gly Gly Gly Asn Asn Gly Gly Gly Asn Asn Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Cys Ser Pro Leu (SEQ ID NO;
12);Pro Gly Gly Asn Asn Asn Asn Pro Pro Pro Ala Thr Thr Ser Gln Trp Thr Pro Pro Pro Ala Gln Thr Ser Ser Asn Pro Pro Pro Thr Gly Gly Gly Gly Gly Asn Thr Leu His Glu Lys (SEQ ID NO;
13);Gly Gly Ser Asn Asn Gly Gly Gly Asn Asn Asn Gly Gly Gly Asn Asn Asn Gly Gly Gly Gly Asn Asn Asn Gly Gly Gly Asn Asn Asn Gly Gly Gly Asn Thr Gly Gly Gly Ser Ala Pro Leu (SEQ ID NO;
14);Val Phe Thr Cys Ser Gly Asn Ser Gly Gly Gly Ser Asn Pro Ser Asn Pro Asn Pro Pro Thr Pro Thr Thr Phe Ile Thr Gln Val Pro Asn Pro Thr Pro Val Ser Pro Pro Thr Cys Thr Val Ala Lys (SEQ ID NO;
15);Pro Ala Leu Trp Pro Asn Asn Asn Pro Gln Gln Gly Asn Pro Asn Gln Gly Gly Asn Asn Gly Gly Gly Asn Gln Gly Gly Gly Asn Gly Gly Cys Thr Val Pro Lys (SEQ ID NO;
16);Pro Gly Ser Gln Val Thr Thr Ser Thr Thr Ser Ser Ser Ser Thr Thr Ser Arg Ala Thr Ser Thr Thr Ser Ala Gly Gly Val Thr Ser Phe Thr Thr Ser Pro Thr Arg Thr Val Thr Ile Pro Gly Gly Ala Ser Thr Thr Ala Ser Tyr Asn (SEQ ID NO;
17);Glu Ser Gly Gly Gly Asn Thr Asn Pro Thr Asn Pro Thr Asn Pro Thr Asn Pro Thr Asn Pro Thr Asn Pro Trp Asn Pro Gly Asn Pro Thr Asn Pro Gly Asn Pro Gly Gly Gly Asn Gly Gly Asn Gly Gly Asn Cys Ser Pro Leu (SEQ ID NO;
18); andPro Ala Val Gln Ile Pro Ser Ser Ser Thr Ser Ser Pro Val Asn Gln Pro Thr Ser Thr Ser Thr Thr Ser Thr Ser Thr Thr Ser Ser Pro Pro Val Gln Pro Thr Thr Pro Ser Gly Cys Thr Ala Glu Arg (SEQ ID NO;
19). - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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44. An isolated and purified DNA sequence which codes for a cellulose- or hemicellulose-degrading enzyme, wherein the enzyme comprises a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, and wherein the carbohydrate binding domain has the following core sequence:
- ##STR12## wherein;
Xaa in position 1 is Trp or Tyr;Xaa in position 2 is Gly or Ala; Xaa in position 7 is Gln, Ile or Asn; Xaa in position 8 is Gly or Asn; Xaa in position 9 is Trp, Phe or Tyr; Xaa in position 10 is Ser, Asn, Thr or Gln; Xaa in position 12 is Pro, Ala or Cys; Xaa in position 13 is Thr, Arg or Lys; Xaa in position 14 is Thr, Cys or Asn; Xaa in position 16 is one of the twenty standard L-α
-amino acids found in proteins;Xaa in position 17 is one of the twenty standard L-α
-amino acids found in proteins;Xaa position 18 is Gly or Pro; Xaa position 19 is Ser, Thr, Phe, Leu or Ala, or is absent; Xaa position 20 is Thr or Lys; Xaa position 22 is Val, Thr, Arg, Glu or Lys; Xaa position 23 is Lys, Gln or Ala; Xaa position 24 is Gln or Ile; Xaa position 26 is Gln, Asp or Ala; Xaa position 27 is Trp, Phe or Tyr; Xaa in position 29 is Tyr, Ser, His or Ala; Xaa in position 32 is Leu, Ile, Gln, Val or Thr; and Xaa in position 33 is one of the twenty standard L-α
-amino acids found in proteins, or is absent;wherein the enzyme is derived from a fungus other than Trichoderma or Phanerochaete. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 73, 74, 75, 76, 77, 78, 79)
- ##STR12## wherein;
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61. An isolated and purified DNA sequence which codes for a cellulose- or hemicellulose-degrading enzyme, wherein the enzyme comprises a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalyactive active domain and the carbohydrate binding domain, and wherein the linking B region has an amino acid sequence selected from the group consisting of:
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Ala Arg Thr Asn Val Gly Gly Gly Ser Thr Gly Gly Gly Asn Asn Gly Gly Gly Asn Asn Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Pro Gly Gly Asn Cys Ser Pro Leu (SEQ ID NO;
12);Pro Gly Gly Asn Asn Asn Asn Pro Pro Pro Ala Thr Thr Ser Gln Trp Thr Pro Pro Pro Ala Gln Thr Ser Asn Pro Pro Pro Thr Gly Gly Gly Gly Gly Asn Thr Leu His Glu Lys (SEQ ID NO;
13);Gly Gly Ser Asn Asn Gly Gly Gly Asn Asn Asn Gly Gly Gly Asn Asn Asn Gly Gly Gly Gly Asn Asn Asn Gly Gly Gly Asn Asn Asn Gly Gly Gly Asn Thr Gly Gly Gly Ser Ala Pro Leu (SEQ ID NO;
14);Val Phe Thr Cys Ser Gly Asn Ser Gly Gly Gly Ser Asn Pro Ser Asn Pro Asn Pro Pro Thr Pro Thr Thr Phe Ile Thr Gln Val Pro Asn Pro Thr Pro Val Ser Pro Pro Thr Cys Thr Val Ala Lys (SEQ ID NO;
15);Pro Ala Leu Trp Pro Asn Asn Asn Pro Gln Gln Gly Asn Pro Asn Gln Gly Gly Asn Asn Gly Gly Gly Asn Gln Gly Gly Gly Asn Gly Gly Cys Thr Val Pro Lys (SEQ ID NO;
16);Pro Gly Ser Gln Val Thr Thr Ser Thr Thr Ser Ser Ser Ser Thr Thr Ser Arg Ala Thr Ser Thr Thr Ser Ala Gly Gly Val Thr Ser Ile Thr Thr Ser Pro Thr Arg Thr Val Thr Ile Pro Gly Gly Ala Ser Thr Thr Ala Ser Tyr Asn (SEQ ID NO;
17);Glu Ser Gly Gly Gly Asn Thr Asn Pro Thr Asn Pro Thr Asn Pro Thr Asn Pro Thr Asn Pro Thr Asn Pro Trp Asn Pro Gly Asn Pro Thr Asn Pro Gly Asn Pro Gly Gly Gly Asn Gly Gly Asn Gly Gly Asn Cys Ser Pro Leu (SEQ ID NO;
18); andPro Ala Val Gln Ile Pro Ser Ser Ser Thr Ser Ser Pro Val Asn Gln Pro Thr Ser Thr Ser Thr Thr Ser Thr Ser Thr Thr Ser Ser Pro Pro Val Gln Pro Thr Thr Pro Ser Gly Cys Thr Ala Gln Arg (SEQ ID NO;
19)wherein the enzyme is derived from a fungus other than Trichoderma or Phanerochaete. - View Dependent Claims (62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 80, 81, 82, 83, 84, 85, 86)
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Specification