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Cancer diagnosis using nucleic acid hybridization

  • US 5,688,641 A
  • Filed: 10/13/1994
  • Issued: 11/18/1997
  • Est. Priority Date: 02/28/1991
  • Status: Expired due to Fees
First Claim
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1. A method of determining, by in situ hybridization, whether a sample containing a test cell from human breast tissue is (a) normal, or (b) cancerous, said method comprising the steps of:

  • contacting the mRNA of said test cell in situ with a nucleic acid probe comprising a nucleotide sequence at least 15 nucleotides in length which is complementary to a portion of the coding sequence or a candidate tumor suppressor gene, said gene being one which is normally expressed in normal cells of human breast tissue; and

    determining whether said probe hybridizes to said mRNA under high stringency conditions selected to permit hybridization of said probe to normal cells of human breast tissue, wherein the failure to hybridize to said mRNA is an indication that said test cell is cancerous;

    provided that said gene encodes connexin 26, connexin 43, preproelafin, CaN19 is protein, or HBp17.

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