Continuous fermentation process for the production of metabolities using a moving filter

• US 5,688,674 A
• Filed: 02/27/1996
• Issued: 11/18/1997
• Est. Priority Date: 09/29/1995
• Status: Expired due to Fees

• Current Assignee: Dreambiogen Company Limited
• Original Assignee: Cha-Yong Choi
• Inventors: Yang, Young-Lyeol, Choi, Cha-Yong
• Primary Examiner(s): Rollins, John W.
• Assistant Examiner(s): TATE, CHRISTOPHER ROBIN
• Application Number:
  US08/607,482
• Time in Patent Office: 630 Days
• Field of Search: 435/161, 435/162, 435/163, 435/165, 435/813, 435/913, 435/940, 435/942
• US Class Current: 435/162
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 435/813 Continuous fermentation
• CPC Class Codes: Y10S 435/913 Aspergillus
• CPC Class Codes: Y10S 435/94 Saccharomyces
• CPC Class Codes: Y10S 435/942 Saccharomyces cerevisiae

Abstract

A metabolite, e.g., ethanol, is continuously produced from low cost carbohydrate substrates by a process which comprises pulverizing the carbohydrate substrate; liquefying and saccharifying the pulverized substrate; continuously fermenting the lique-saccharified substrate in a fermentor equipped with a moving filter, in the presence of flocculent biological cells maintained at a concentration ranging from 90 to 160 g/l by using the moving filter and a culture medium to produce a fermentation product mixture; and recovering the desired metabolite from the fermentation product mixture.

Claims

1. A continuous process for producing a metabolite from a carbohydrate substrate which comprises the steps of:

(A) pulverizing the carbohydrate substrate;

(B) liquefying and saccharifying the pulverized substrate;

(C) continuously fermenting the lique-saccharified substrate in a fermentor, in the presence of biological cells and a culture medium to produce a fermentation product mixture; and

(D) recovering the metabolite from the fermentation product mixture;

wherein the fermentor is equipped with a moving filter having an opening size ranging from 50 to 2,000 mesh and the biological cells are flocculent cells maintained at a concentration ranging from 90 to 160 g/l by the moving filter during the fermentation step.

2. The process of claim 1, wherein the liquefaction-saccharification in step (B) is carried out by adding water and enzymes to the pulverized substrate and maintaining the resultant mixture at a temperature ranging from 50°

to 95°

C.

3. The process of claim 2, wherein the lique-saccharified substrate obtained from step (B) has a glucose concentration ranging from 140 to 300 g/l and solid particles content of less than 60% (v/T), with the solid particles having a size distribution ranging from 1 to 1,000 μ

m.

4. The process of claim 2, wherein the enzyme employed in step (B) is selected from the group consisting of amylase, glucoamylase, cellulase, cellobiase and a mixture thereof.

5. The process of claim 1, wherein the lique-saccharified substrate obtained from step (B) is passed through a solid/liquid separator so as to remove a major portion of the solid particles therefrom prior to feeding the substrate to the fermentor.

6. The process of claim 5, wherein the lique-saccharified substrate has solid particles content of less than about 20% (v/v).

7. The process of claim 1, wherein the filter is of a screen or membrane type.

8. The process of claim 1, wherein the recovery in step (D) is carried out by removing bled cells from the fermentation product mixture to obtain a supernatant thereof and separating the metabolite from the supernatant.

9. The process of claim 8, wherein the cells removed are recycled to the fermentor.

10. The process of claim 1, wherein the metabolite is ethanol.

11. The process of claim 10, wherein the flocculent cells employed in step (C) are provided from a yeast.

12. The process of claim 11, wherein said yeast is selected from Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomyces uvarum or a mixture thereof.

13. The process of claim 10, wherein the culture medium employed in step (C) includes a nitrogen containing compound selected from the group consisting of ammonium phosphate monohydride, ammonium phosphate dihydride, ammonium sulfate, ammonium acetate, ammonium nitrate, urea, yeast extract, peptone and a mixture thereof and additional compounds selected from the group consisting of sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium chloride, calcium chloride, ferrous sulfate, magnesium sulfate and a mixture thereof.

14. The process of claim 10, wherein the fermentation in step (C) is carried out at a temperature ranging from 4°

to 40°

C., at a pH ranging from 3.5 to 9, at a filter rotation rate of 1,000 rpm or less and at a dilution rate of the substrate ranging from 0.5 to 2.0 hr^-1.

15. The process of claim 14, wherein the fermentation is carried out at a temperature ranging from 15°

to 35°

C., at a pH ranging from 4 to 8 and at a filter rotation rate of below about 500 rpm.