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Pluripotential embryonic cells and methods of making same

  • US 5,690,926 A
  • Filed: 03/25/1994
  • Issued: 11/25/1997
  • Est. Priority Date: 10/08/1992
  • Status: Expired due to Term
First Claim
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1. An isolated non-murine mammalian pluripotential cell wherein said cell exhibits the following characteristics:

  • (a) can be maintained on feeder layers for at least 20 passages; and

    (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and

    wherein said cell is derived from a primordial germ cell by the process of;

    (1) culturing a non-murine mammalian primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor;

    (2) selecting cells that have characteristics (a) and (b), above, and(3) isolating said non-murine pluripotential cell.

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