Pluripotential embryonic cells and methods of making same
First Claim
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1. An isolated non-murine mammalian pluripotential cell wherein said cell exhibits the following characteristics:
- (a) can be maintained on feeder layers for at least 20 passages; and
(b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and
wherein said cell is derived from a primordial germ cell by the process of;
(1) culturing a non-murine mammalian primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor;
(2) selecting cells that have characteristics (a) and (b), above, and(3) isolating said non-murine pluripotential cell.
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Abstract
The claimed invention is directed towards non-murine pluripotential cells that have the ability to be passaged in vitro for at least 20 passages and which differentiate in culture into a variety of tissues. The scope of the claimed cells includes any non-murine ES cells and particular claims are drawn to human pluripotential cells.
173 Citations
7 Claims
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1. An isolated non-murine mammalian pluripotential cell wherein said cell exhibits the following characteristics:
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(a) can be maintained on feeder layers for at least 20 passages; and (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and
wherein said cell is derived from a primordial germ cell by the process of;(1) culturing a non-murine mammalian primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (2) selecting cells that have characteristics (a) and (b), above, and (3) isolating said non-murine pluripotential cell. - View Dependent Claims (2, 3, 4, 5)
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6. An isolated human pluripotential cell wherein said cell exhibits the following characteristics:
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(a) can be maintained on feeder layers for at least 20 passages; and (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and
wherein said cell is derived from a human primordial germ cell by the process of;(1) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (2) selecting cells that have characteristics (a) and (b), above, and (3) isolating said human pluripotential cell.
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7. A composition comprising:
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(A) a human pluripotential cell derived from a primordial germ cell wherein said cell exhibits the following characteristics; (1) can be maintained on feeder layers for at least 20 passages; and (2) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and
wherein said cell is derived from a primordial germ cell by the process of;(a) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (b) selecting cells that have characteristics (a) and (b), above, and (c) isolating said human pluripotential cell; and (B) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor, each in amounts sufficient to permit continued proliferation of said cell.
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Specification