Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase

• US 5,691,178 A
• Filed: 06/07/1995
• Issued: 11/25/1997
• Est. Priority Date: 03/24/1988
• Status: Expired due to Term

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novo Nordisk A/S
• Inventors: Schulein, Martin, Levring, Kirsten B.o slashed.egh
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US08/487,917
• Time in Patent Office: 902 Days
• Field of Search: 435/209, 252/174.12, 252/DIG. 12, 510/392, 510/320, 510/530
• US Class Current: 435/209
• CPC Class Codes: C11D 3/38645 containing cellulase
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: D06M 16/003 with enzymes or microorganisms
• CPC Class Codes: D06M 2101/06 cellulosic
• CPC Class Codes: D06M 2200/50 Modified hand or grip properties; Softening compositions

Abstract

A cellulase preparation useful for reducing the harshness of cotton-containing fabrics and for reducing the rate at which such fabrics become harsh comprises about 40% or more (based on the total protein content) of an endoglucanase component with a pH optimum of about 7.5-10.0 with a high CMC-endoase activity and affinity towards cellulose and essentially no cellobiohydrolase activity. The cellulase composition is endogenous to a strain of Humicola, Myceliophthora, or Fusarium.

Claims

1. A cellulase preparation which comprises a substantially homogeneous endoglucanase component having

(a) a pH optimum of about 7.5-10.0;

(b) a carboxymethyl cellulose (CMC) endoase activity of at least about 10 CMC-endoase units per mg of total protein;

(c) a cellulose affinity viscosity unit (CAVU) activity of at least about 50% at a pH of 9.0; and

(d) essentially no cellobiohydrolase activity;

wherein the cellulase preparation is endogenous to a strain of Humicola, Myceliophthora, or Fusarium and wherein the endoglucanase component is present in an mount of at least about 50% by weight of the total cellulase content of the cellulase preparation.

2. The cellulase preparation according to claim 1, which is endogenous to Humicola.

3. The cellulase preparation according to claim 2, which is endogenous to Humicola insolens.

4. The cellulase preparation according to claim 3, which is endogenous to Humicola insolens, DSM 1800.

5. The cellulase preparation according to claim 1, which is endogenous to Fusarium.

6. The cellulase preparation according to claim 5, which is endogenous to Fusarium oxysporum.

7. The cellulase preparation according to claim 1, which is endogenous to Myceliophthora.

8. The cellulase preparation according to claim 7, which is endogenous to Myceliophthora thermophila.

9. The cellulase preparation according to claim 1, wherein the endoglucanase component is present in an amount of at least about 90% by weight of the total cellulase content of the cellulase preparation.

10. The cellulase preparation according to claim 1, whereto the endoglucanase component has a CMC-endoase activity of at least about 15 CMC-endoase units per mg of total protein.

11. The cellulase preparation according to claim 10, wherein the endoglucanase component has a CMC-endoase activity of at least about 50 CMC-endoase units per mg of total protein.

12. The cellulase preparation according to claim 11, wherein the endoglucanase component has a CAVU activity in excess of about 65%.

13. The cellulase preparation according to claim 12, wherein the endoglucanase component has a CAVU activity in excess of about 75%.

14. The cellulase preparation according to claim 3, wherein the endoglucanase component further has

(a) a molecular weight of about 65 kilodaltons as determined by denaturing gel electrophoresis;

and

(b) an isoelectric point of about 8.5-9.5.

15. A detergent additive comprising the cellulase preparation of claim 1 in the form of a non-dusting granulate, stabilized liquid or protected enzyme.

16. A detergent composition comprising the cellulase preparation of claim 1 and a surfactant.

17. The detergent composition of claim 16, wherein the cellulase preparation exhibits a CMC-endoase activity of about 0.3-400 CMC-endoase units per gram of detergent.

18. The detergent composition of claim 16, further comprising a second enzyme.

19. The detergent composition of claim 18, wherein the second enzyme is selected from the group consisting of protease, lipase, and amylase.