Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase
First Claim
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1. A cellulase preparation which comprises a substantially homogeneous endoglucanase component having(a) a pH optimum of about 7.5-10.0;
- (b) a carboxymethyl cellulose (CMC) endoase activity of at least about 10 CMC-endoase units per mg of total protein;
(c) a cellulose affinity viscosity unit (CAVU) activity of at least about 50% at a pH of 9.0; and
(d) essentially no cellobiohydrolase activity;
wherein the cellulase preparation is endogenous to a strain of Humicola, Myceliophthora, or Fusarium and wherein the endoglucanase component is present in an mount of at least about 50% by weight of the total cellulase content of the cellulase preparation.
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Abstract
A cellulase preparation useful for reducing the harshness of cotton-containing fabrics and for reducing the rate at which such fabrics become harsh comprises about 40% or more (based on the total protein content) of an endoglucanase component with a pH optimum of about 7.5-10.0 with a high CMC-endoase activity and affinity towards cellulose and essentially no cellobiohydrolase activity. The cellulase composition is endogenous to a strain of Humicola, Myceliophthora, or Fusarium.
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19 Claims
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1. A cellulase preparation which comprises a substantially homogeneous endoglucanase component having
(a) a pH optimum of about 7.5-10.0; -
(b) a carboxymethyl cellulose (CMC) endoase activity of at least about 10 CMC-endoase units per mg of total protein; (c) a cellulose affinity viscosity unit (CAVU) activity of at least about 50% at a pH of 9.0; and (d) essentially no cellobiohydrolase activity; wherein the cellulase preparation is endogenous to a strain of Humicola, Myceliophthora, or Fusarium and wherein the endoglucanase component is present in an mount of at least about 50% by weight of the total cellulase content of the cellulase preparation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification