Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure
First Claim
Patent Images
1. A composition for effecting wound closure, comprising keratinocytes supported on a hydrophilic membrane, wherein said membrane has a sufficiently small pore size that reduces or inhibits the ability of bacteria to infect the wound.
1 Assignment
0 Petitions
Reexamination
Accused Products
Abstract
The present invention provides keratinocytes supported on a hydrophilic membrane, the composition being useful as a skin graft to effect closure of a wound. The invention also provides methods of preparing a keratinocyte-containing membrane suitable for effecting wound closure. In addition, the invention provides methods of effecting closure of wound comprising contacting the wound with a keratinocyte-containing membrane.
99 Citations
21 Claims
- 1. A composition for effecting wound closure, comprising keratinocytes supported on a hydrophilic membrane, wherein said membrane has a sufficiently small pore size that reduces or inhibits the ability of bacteria to infect the wound.
- 4. A method of effecting wound closure in a patient having a wound, comprising contacting the wound with keratinocytes supported on a hydrophilic membrane, wherein said membrane has a sufficiently small pore size that reduces or inhibits the ability of bacteria to infect the wound.
-
9. A method of obtaining a composition for effecting wound closure, comprising keratinocytes supported on a hydrophilic membrane, comprising the steps of:
-
a. tautly mounting the hydrophilic membrane in a vessel wherein said membrane has a sufficiently small pore size that reduces or inhibits the ability of bacteria to infect the wound; b. seeding said membrane with a suitable number of the keratinocytes; c. thereafter, incubating said keratinocyte-seeded membrane under conditions that allow proliferation of said keratinocytes; and d. terminating said incubating step, wherein said keratinocytes supported on said membrane is obtained. - View Dependent Claims (10, 11, 14, 15, 16, 17)
-
Specification