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Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support

  • US 5,695,926 A
  • Filed: 06/07/1994
  • Issued: 12/09/1997
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. Procedure for the detection of a single-stranded polynucleotide target sequence in a sample, comprising the steps consisting of:

  • a) incubating the sample;

    i) with a capture probe adsorbed to a hydrophobic solid support, wherein said capture probe is an oligonucleotide having from 11 to 19 nucleotides and said capture probe is not derivatized with a moiety to facilitate adsorption, andii) with a detection probe labelled with a non-radioactive marker, said capture probe and said detection probe being capable of hybridizing with two non-overlapping regions of said target sequence,b) washing said solid support in order to remove material not bound to said support by hybridization, andc) determining whether said marker is bound to said support, the presence of said marker indicating the presence of said single-stranded polynucleotide sequence in said sample.

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