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Reagent and method for the detection of a nucleotide sequence with signal amplification

  • US 5,695,936 A
  • Filed: 06/14/1995
  • Issued: 12/09/1997
  • Est. Priority Date: 09/15/1993
  • Status: Expired due to Term
First Claim
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1. A method for assaying at least one nucleic acid sequence of interest in a nucleic acid target, with signal amplification, comprising:

  • contacting a sample containing said nucleic acid target with(i) a linear backbone polymer reagent which provides signal amplification,wherein said reagent is soluble in aqueous media and comprises monomeric units forming a backbone chain of carbon-to-carbon covalent bonds wherein at least one monomeric unit has an oligonucleotide lateral substituent that hybridizes with said sequence of interest and hybridizes with a tracer-labeled probe, and wherein said reagent has at least two oligonucleotide lateral substituents per polymer molecule,(ii) a single species of tracer-labeled probe that hybridizes to said oligonucleotide lateral substituent on said reagent,(iii) a solid support, and(iv) means for immobilizing said nucleic acid target on said solid support, under conditions that permit hybridization;

    thereby forming a complex comprising, in order, said solid support, said nucleic acid target, said reagent, and said tracer-labeled probe; and

    assaying for presence of said tracer-labeled probe immobilized on said solid support.

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