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Reagent for endotoxin assay and method for endotoxin assay using the same

  • US 5,695,948 A
  • Filed: 06/11/1996
  • Issued: 12/09/1997
  • Est. Priority Date: 02/26/1993
  • Status: Expired due to Term
First Claim
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1. A method for specifically assaying for endotoxin in a sample comprising the steps of:

  • (A) adding aprotinin to a horseshoe crab amebocyte lysate reagent,(B) adding the resulting lysate reagent of step (A) to the sample, and(C) assaying for endotoxin in the resulting mixture of Step (B),wherein said aprotinin is added to the lysate reagent in Step (A) in an amount sufficient to completely inhibit activation of factor G present in the horseshoe crab amebocyte lysate reagent.

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