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Neurospora expression system

  • US 5,695,965 A
  • Filed: 06/02/1992
  • Issued: 12/09/1997
  • Est. Priority Date: 03/13/1990
  • Status: Expired due to Term
First Claim
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1. A method of producing a mammalian peptide in a Neurospora fungus host, said method comprising:

  • transforming by DNA uptake Neurospora spheroplasts with (1) a DNA construct comprising as operably linked components a constitutive promoter functional in said fungus host, a first DNA sequence encoding a mammalian peptide under the transcriptional regulation of said promoter and a transcriptional termination regulatory region functional in said host, and (2) a second DNA sequence comprising a marker gene which is expressed in said host;

    selecting for transformed host cells comprising said construct by means of said marker gene;

    identifying stably transformed host cells; and

    growing said stably transformed host cells whereby said first DNA sequence is expressed, and said cells produce said mammalian peptide.

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