Thermophilic fungal expression system
First Claim
1. A recombinant fungal host strain in which said strain is obtained from a member of the genus Acremonium, Corynascus, Thielavia, Myceliophthora, Thermoascus, Sporotrichum, Chaetomium, Ctenomyces, or Scytalidium and in which said strain produces at least 75 g/l of biomass concentration, dry weight, in tank fermentation.
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Accused Products
Abstract
The present invention relates to recombinant thermophilic host cells comprising a nucleic acid sequence encoding a heterologous protein, and a method of producing recombinant protein utilizing same. The recombinant hosts of the present invention provide a better morphology in tank fermentations than many known fungal host cells, such as Aspergillus, which morphology results in lower viscosity levels, and therefore improved productivity.
17 Citations
3 Claims
- 1. A recombinant fungal host strain in which said strain is obtained from a member of the genus Acremonium, Corynascus, Thielavia, Myceliophthora, Thermoascus, Sporotrichum, Chaetomium, Ctenomyces, or Scytalidium and in which said strain produces at least 75 g/l of biomass concentration, dry weight, in tank fermentation.
Specification
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- Resources
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Current AssigneeNovozymes
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Original AssigneeNOVO Nordisk Biotech Incorporated (Novo Nordisk A/S)
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InventorsJensen, Ejner Bech, Boominathan, Karuppan Chettiar
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Primary Examiner(s)KETTER, JAMES S
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Application NumberUS08/456,843Time in Patent Office922 DaysField of Search435/69.1, 435/254.11US Class Current435/254.11CPC Class CodesC12N 15/80 for fungiC12N 9/248 XylanasesY10S 435/911 using fungi
