Gene delivery by microneedle injection
First Claim
1. A method for introducing genetic material into a cell the method comprising the step ofrepeatedly injecting the genetic material into a plurality of cells of a target tissue at an in situ site on a human or non-human animal with a microneedle, whereby a change in the animal attributable to the delivery of the genetic material is detectable.
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Accused Products
Abstract
Direct gene transfer of genetic material into an external or internal target cell site ("microseeding"), in optional combination with a wound treatment chamber, are particularly effective as a way of obtaining long term expression of native or non-native polypeptides in a host. A wide variety of proteins and materials can be expressed, either for secretion into the general blood and lymphatic system, or to alter the properties of the protein, for example, to not express proteins eliciting an immune response. The use of the optional wound chamber system for gene transfer to skin target sites also allows non-invasive assessment of the success of transfer by assaying for the presence of the expressed protein in wound fluid, in contrast to the prior art use of invasive techniques, such as biopsies, in order to achieve the same assessment of early expression.
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Citations
21 Claims
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1. A method for introducing genetic material into a cell the method comprising the step of
repeatedly injecting the genetic material into a plurality of cells of a target tissue at an in situ site on a human or non-human animal with a microneedle, whereby a change in the animal attributable to the delivery of the genetic material is detectable.
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5. A method for delivering genetic material into a human or non-human subject, the method comprising the steps of:
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contacting a tip of a microneedle with a solution comprising the genetic material; penetrating the tip repeatedly into a site on a surface of a target tissue at an in situ site on the subject, to a depth in the tissue selected to allow contact between the tip and a plurality of target cells that can take up the genetic material, until a sufficient amount of the genetic material is delivered to the target cells such that a change in the subject attributable to the delivery of the genetic material is detectable. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for delivering genetic material into a target tissue of a subject, the method comprising the steps of:
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contacting a tip of a microneedle with a solution comprising the genetic material; penetrating the tip repeatedly into a site on a surface of the target tissue, to a depth selected to allow contact between the tip and a plurality of target cells that can take up the genetic material, until a sufficient amount of the genetic material is delivered to the target cells such that a change in the subject attributable to the delivery of the genetic material is detectable, wherein the target tissue is selected from the group consisting of skin cells and a wound, the method further comprising the step of covering the target cells with a protective chamber.
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Specification