Chemiresistor urea sensor

• US 5,698,083 A
• Filed: 08/18/1995
• Issued: 12/16/1997
• Est. Priority Date: 08/18/1995
• Status: Expired due to Fees

• Current Assignee: Lawrence Livermore National Security LLC (Government of the United States of America)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Glass, Robert S.
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/517,011
• Time in Patent Office: 851 Days
• Field of Search: 204/403, 204/406, 204/415, 204/416, 204/418, 422/68.1, 422/82.02, 422/82.01, 422/98, 435/4, 435/12, 435/288, 435/291, 128/637, 436/108, 436/114, 436/149, 436/151
• US Class Current: 204/403.03
• CPC Class Codes: C12Q 1/58 involving urea or urease
• CPC Class Codes: Y10T 436/171538 Urea or blood urea nitrogen
• CPC Class Codes: Y10T 436/176152 Total nitrogen determined

Abstract

A sensor to detect and quantify urea in fluids resulting from hemodialysis procedures, and in blood and other body fluids. The sensor is based upon a chemiresistor, which consists of an interdigitated array of metal fingers between which a resistance measured. The interdigitated array is fabricated on a suitable substrate. The surface of the array of fingers is covered with a coating containing the enzyme urease which catalyzes the hydrolysis of urea to form the ammonium ion, the bicarbonate ion, and hydroxide-chemical products which provide the basis for the measured signal. In a typical application, the sensor could be used at bedside, in conjunction with an appropriate electronics/computer system, in order to determine the hemodialysis endpoint. Also, the chemiresistor used to detect urea, can be utilized with a reference chemiresistor which does not contain urease, and connected in a differential measurement arrangement, such that the reference chemiresistor would cancel out any fluctuations due to background effects.

Claims

1. A chemiresistor device in combination with a urea monitor, said chemiresistor device comprising:

a substrate,

an insulating layer deposited on at least one surface of the substrate,

at least a patterned layer of conductive material including at least one set of interdigitated members deposited on said insulating layer, and

a layer of encapsulation material deposited on at least the interdigitated members,

said layer of encapsulated material being composed of a sol-gel and including an immobilized bioactive component composed of urease,

said urea monitor comprising,

a housing having said chemiresistor device operatively mounted therein, and

electronic means and readout means therefore,

said housing being provided with means for inserting a test strip into said housing for providing a reading of urea conditions via said readout means.

2. The combination of claim 1, wherein said layer of conductive material includes a plurality of sets of interdigitated members, and wherein said layer of material encapsulation on at least one of said plurality of sets of interdigitated members includes the bioactive component urease.

3. The combination of claim 1, additionally including a layer of adhesive material under the layer of conductive material.

4. The combination of claim 1, wherein said substrate is composed of material selected from the group consisting of silicon, aluminum oxide, polyimide (KAPTON), ceramic materials, and polymeric materials.

5. The combination of claim 1, wherein said insulating layer is composed of material selected from the group consisting of silicon nitride and silicon dioxide.

6. The combination of claim 1, wherein said layer of conductive material is composed of material selected from the group consisting of gold, platinum, iridium, carbon, and conductive, inert materials.

7. The combination of claim 1, wherein said layer of conductive material has a thickness of 0.1 to 1.0 μ

m, wherein said interdigitated members have a width of 10-15 μ

m, a length of 200 μ

m to 4.8 mm, and are separated from one another by a distance of 10-15 μ

m.

8. The combination of claim 7, wherein said set of interdigitated members comprises a pair of opposing side strips and a plurality of members attached to each of said side strips, and wherein an end of the members attached to one side strip is spaced from an opposing side strip by a distance of 10-15 μ

m.

9. The combination of claim 1, wherein said test strip includes an electrical contact section and a sample section.

10. An apparatus for detecting/monitoring urea, including:

a chemiresistor sensor containing an array of interdigitated fingers covered by a coating composed of a sol-gel containing urease,

a reference chemiresistor, containing an array of interdigitated fingers without a coating containing urease,

said chemiresistor sensor and said reference chemiresistor being operatively connected to a differential amplifier,

said differential amplifier being operatively connected to a microcomputer,

a function generator operatively connected to at least one of said chemiresistor sensor and said reference chemiresistor, and

a display/recorder mechanism operatively connected to said microcomputer.