European vaccine strains of the Porcine Reproductive Respiratory Syndrome virus (PRRSV)

• US 5,698,203 A
• Filed: 04/11/1995
• Issued: 12/16/1997
• Est. Priority Date: 04/11/1994
• Status: Expired due to Term

• Current Assignee: Intervet International BV (Merck & Co., Inc.)
• Original Assignee: Akzo Nobel N.V. (Nouryon BV)
• Inventors: Ohlinger, Volker, Weiland, Emilie, Visser, Nicolaas, Van Woensel, Petrus Alphonsus Maria
• Primary Examiner(s): Feisee, Lila
• Assistant Examiner(s): Reeves, Julie E.
• Application Number:
  US08/419,781
• Time in Patent Office: 980 Days
• Field of Search: 424/218.1, 424/147.1, 530/388.3, 530/390.1, 530/389.4, 435/236, 435/239, 435/7.1
• US Class Current: 424/218.1
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/552 Veterinary vaccine
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

The present invention is concerned with European strains of the Porcine Reproductive Respiratory Syndrome (PRRS) virus, which are attenuated, and show a characteristic reaction pattern with two monoclonal antibodies against wild-type PRRSV.

The invention also relates to vaccines for the protection of pigs against PRRS, to monoclonal antibodies reactive with PRRS virus and monoclonal antibodies specifically non-reactive with the attenuated strains.

Claims

1. A Porcine Reproductive and Respiratory Syndrome (PRRS) virus, of a European serotype, said virus being an attenuated virus reactive with monoclonal antibody A27, as produced by hybridoma A27 deposited with the Collection Nationale De Cultures De Microorganismes (CNCM) of the Institut Pasteur at Paris, France under accession number I-1401, but not reactive with monoclonal antibody A35, as produced by hybridoma A35 deposited with the Institut Pasteur at Paris, France under accession number I-1402.

2. The attenuated PRRS virus according to claim 1, said virus selected from the group consisting of the strains deposited with the Institut Pasteur at Paris, France under accession numbers I-1388 and I-13870 and further passages of I-1388 and I-1387.

3. A vaccine for the protection of pigs against Porcine Reproductive and Respiratory Syndrome (PRSS), comprising an immunogenically effective amount of a live virus according to claim 1 and a pharmaceutically acceptable carrier.

4. The vaccine according to claim 3, comprising 10-10⁷ virus particles.

5. A vaccine for the protection of pigs against Porcine Reproductive and Respiratory Syndrome (PRRS), comprising an immunogenically effective amount of a virus according to claim 1 in an inactivated form and a pharmaceutically acceptable carrier.

6. The vaccine according to claim 5, wherein the virus has a pre-inactivation titre of 10³ -10¹⁰ virus particles.

7. The vaccine according to claim 3, comprising an adjuvant.

8. The vaccine according to claim 5, comprising an adjuvant.