Method for quantification of biological material in a sample
First Claim
1. A method for detection of the amount of a biological material in a sample, comprising the steps of:
- liquefying said sample if necessary, and pouring the liquefied sample into an incubation plate comprising a generally flat horizontal surface, said surface defining a plurality of at least twenty recessed wells, each well being adapted to hold an aliquot of liquid and being sized and shaped, and formed of a material suitable to hold said aliquot within each said well by surface tension or by gelling of said liquid, said plate further comprising a landing pad area of size sufficient to hold the liquefied sample prior to dispersal of the sample to said wells;
pouring off any excess liquid from said incubation plate, andincubating said incubation plate until the presence or absence of said biological material in one or more of said wells is determined so that the mount of said biological material can be determined.
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Abstract
Method for detection of a biological material in a sample. The method includes the steps of liquifying the sample (if necessary) and pouring the liquified sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquified sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.
94 Citations
22 Claims
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1. A method for detection of the amount of a biological material in a sample, comprising the steps of:
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liquefying said sample if necessary, and pouring the liquefied sample into an incubation plate comprising a generally flat horizontal surface, said surface defining a plurality of at least twenty recessed wells, each well being adapted to hold an aliquot of liquid and being sized and shaped, and formed of a material suitable to hold said aliquot within each said well by surface tension or by gelling of said liquid, said plate further comprising a landing pad area of size sufficient to hold the liquefied sample prior to dispersal of the sample to said wells; pouring off any excess liquid from said incubation plate, and incubating said incubation plate until the presence or absence of said biological material in one or more of said wells is determined so that the mount of said biological material can be determined. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A sterile incubation plate for determination of the mount of a biological material, comprising a generally flat horizontal surface, said surface defining a plurality of at least twenty recessed wells, each said well being adapted to hold an aliquot of liquid and being sized and shaped, and formed of a material suitable to hold said aliquot within each said well by surface tension and wherein said plate further comprises a landing pad adapted to hold a sample prior to aliquoting of said sample to said wells,
wherein said incubation plate will not provide any positive response for said biological material in the absence of a said biological material present in a sample applied to said plate.
- 14. The sterile incubation plate of 12, wherein said plate further comprises a pour spout.
Specification