Flow sorter with video-regulated droplet spacing

• US 5,700,692 A
• Filed: 09/27/1994
• Issued: 12/23/1997
• Est. Priority Date: 09/27/1994
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Sweet, Richard G.
• Primary Examiner(s): Le, Long V.
• Application Number:
  US08/312,592
• Time in Patent Office: 1,183 Days
• Field of Search: 436/63, 436/50, 436/43, 436/52, 436/164, 436/55, 436/807, 436/10, 422/68.1, 422/73, 422/81, 422/105, 209/3.1, 209/3.2, 209/552, 209/576, 209/577, 209/587, 209/638, 209/588, 209/939
• US Class Current: 436/50
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: Y10S 209/939 Video scanning
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/12 Condition responsive control

Abstract

A droplet-deflection flow sorter images a series of droplets, analyzes the image to determine droplet spacing, and alters flow velocity to achieve a desired spacing. Cells in a suspension are forced by gas pressure into a narrow conduit to serialize them and then out a nozzle that includes a "window" that allows cells of interest to be characterized. Droplets breaking off from the exiting jet are imaged by a video system including a strobed light source and a video camera. Droplet spacing is determined by locating the centers of gravity of the droplets. The centers of gravity are located by processing the droplet images to produce line segments corresponding to volumetric droplet slices, with the relative volumes of the slices being proportional to the squares of the line segment lengths. This approach determines droplet center positions and thus spacing more accurately than non-imaging methods, especially with aspherical droplets and droplets with satellites. The actual droplet spacing is compared to desired spacing, and the pressure regulator is adjusted accordingly. Synchronously, voltage is applied to droplets of interest. The droplets pass between a pair of deflection plates with a potential difference between them, so that charged droplets containing cells of interest are deflected and gathered while uncharged droplets not containing such cells are not deflected.

Claims

1. A system for sorting particles in a suspension comprising:

flow means for serializing said particles in a stream having a flow velocity;

a droplet generator for generating droplets from said stream, said droplets having a common initial trajectory, each of said droplets being formed from a corresponding stream segment;

detection means for providing a characterization of the contents of each of said stream segments;

charging means for applying a time-varying electric field to said stream so that said droplets are charged as a function of characterization of the contents of the corresponding stream segments;

deflection means for deflecting said droplets so that their post-deflection trajectories are a function of their respective charges;

collection means for collecting said droplets in groups having common post-deflection trajectories;

imaging means for obtaining images, each of said images including subimages of at least two droplets;

image processing means for determining the deviation in the actual spacing of the centers of gravity for the droplets represented in each of said images from a target spacing between droplet centers of gravity; and

flow velocity means for adjusting the flow velocity of said stream as a function said deviation so as to minimize said deviation.

2. A system as recited in claim 1 wherein said image processing means locates the center of gravity for a droplet by equating it with the point in the respective droplet subimage which would be the center of gravity assuming that the respective droplet has cylindrical symmetry about an axis along said common initial trajectory.

3. A method of sorting particles in a suspension comprising the steps of:

serializing said particles in a stream including said suspension, said stream having a flow velocity;

characterizing segments of the stream according to their particle contents so as to provide a characterization of each of said segments;

transporting each segment to a breakoff point;

applying a time-varying electric field to said stream so that the voltage applied is at least in part a function of said characterization of the segment at the breakoff point;

breaking off the segment at the breakoff point so that it forms a droplet having a charge that is a function of the characterization of the respective segment, said droplet having a trajectory;

deflecting the droplets by an angle that is a function of their respective charges;

collecting the droplets so that they are grouped as a function of their deflections;

acquiring an image of a subseries of said droplets after breakoff but before collection;

determining the deviation of the spacing of the centers of gravity of droplets in said image from a target droplet spacing; and

adjusting flow velocity of said stream to reduce any discrepancy between said target spacing of the centers of gravity of droplets and subsequently determined actual spacings of the centers of gravity of droplets.

4. A method as recited in claim 3 wherein said determining step involves locating the center of gravity of a droplet by equating it with the image point that would be the center of gravity of the droplet assuming cylindrical symmetry about its trajectory.