Gel compositions prepared from crosslinkable polysaccharides, polycations and/or lipids and uses therefor
First Claim
1. A biocompatible gel containing entrapped mammalian cells produced by:
- a) chemically modifying a biocompatible material having a reactive functionality thereon with a reactive species capable of free radical polymerization to form a modified biocompatible material having the formula;
space="preserve" listing-type="equation">A--X whereinA is a polysaccharide, polycation or lipid;
X is a moiety containing a carbon-carbon double bond or triple bond capable of free radical polymerization, wherein X is not a methylol amide; and
A and X are linked covalently through linkages selected from the group consisting of ester, ether, thioether, disulfide, amide, secondary amines, tertiary amines, direct C--C linkages, sulfate esters, sulfonate esters, phosphate esters, urethanes, and carbonates; and
b) contacting said modified biocompatible material with a free radical initiating system under free radical producing conditions in an aqueous media that contains mammalian cells and is free of organic solvents, wherein said free radical producing conditions are not detrimental to the viability of said mammalian cells.
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Abstract
The present invention relates to a new form of biocompatible materials (e.g., lipids, polycations, polysaccharides) which are capable of undergoing free radical polymerization, e.g., by using certain sources of light; methods of modifying certain synthetic and naturally occurring biocompatible materials to make polymerizable microcapsules containing biological material coated with said polymerizable materials, composites of said polymerizable materials, methods of making microcapsules and encapsulating biological materials therein, and apparatus for making microcapsules containing biological cells (particularly islets of Langerhans) coated with polymerizable alginate or with a composite thereof (e.g., alginate and PEG). The present invention also relates to drug delivery systems relating to the foregoing, as well as bioadhesives and wound dressings made utilizing the foregoing technology.
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Citations
20 Claims
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1. A biocompatible gel containing entrapped mammalian cells produced by:
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a) chemically modifying a biocompatible material having a reactive functionality thereon with a reactive species capable of free radical polymerization to form a modified biocompatible material having the formula;
space="preserve" listing-type="equation">A--Xwherein A is a polysaccharide, polycation or lipid; X is a moiety containing a carbon-carbon double bond or triple bond capable of free radical polymerization, wherein X is not a methylol amide; and A and X are linked covalently through linkages selected from the group consisting of ester, ether, thioether, disulfide, amide, secondary amines, tertiary amines, direct C--C linkages, sulfate esters, sulfonate esters, phosphate esters, urethanes, and carbonates; and b) contacting said modified biocompatible material with a free radical initiating system under free radical producing conditions in an aqueous media that contains mammalian cells and is free of organic solvents, wherein said free radical producing conditions are not detrimental to the viability of said mammalian cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification