Labeling nucleic acids
First Claim
Patent Images
1. A method for modifying a nucleic acid comprising:
- a. contacting a nucleic acid with a pro-thiol aryl azide having the structure;
##STR40## and salts thereof wherein, R1 to R4, which may be the same or different, are selected from the group consisting of H, alkyl, nitro, hydroxy, halide, carboxylic acid, methoxy, cyano, and amino groups;
wherein E is a chain of C1 -C20 having chain constituents selected from the group consisting of alkyl, alkenyl, alkynyl and heteroatoms, said chain constituents having substituents selected from the group consisting of H, C1 -C6 alkyl, aryl, ester, ether, amine, amide and halo groups; and
wherein Y is a protecting group for protecting the sulfur moiety from chemical reaction and is removable so as to generate a thiol moiety; and
b. subjecting the nucleic acid and the pro-thiol aryl azide to activation conditions sufficient to cause reaction therebetween resulting in modifying the nucleic acid.
1 Assignment
0 Petitions
Accused Products
Abstract
Reagents and methods for multi-step labeling of nucleic acids allow the addition of relatively insoluble or unstable labels to nucleic acid in the final step. Nucleic acids can be stored as a stable intermediate capable of reacting with a label conjugated to a thiol-reactive group.
35 Citations
9 Claims
-
1. A method for modifying a nucleic acid comprising:
-
a. contacting a nucleic acid with a pro-thiol aryl azide having the structure;
##STR40## and salts thereof wherein, R1 to R4, which may be the same or different, are selected from the group consisting of H, alkyl, nitro, hydroxy, halide, carboxylic acid, methoxy, cyano, and amino groups;wherein E is a chain of C1 -C20 having chain constituents selected from the group consisting of alkyl, alkenyl, alkynyl and heteroatoms, said chain constituents having substituents selected from the group consisting of H, C1 -C6 alkyl, aryl, ester, ether, amine, amide and halo groups; and wherein Y is a protecting group for protecting the sulfur moiety from chemical reaction and is removable so as to generate a thiol moiety; and b. subjecting the nucleic acid and the pro-thiol aryl azide to activation conditions sufficient to cause reaction therebetween resulting in modifying the nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
-
8. A labeling kit for labeling a nucleic acid comprising:
-
a) a pro-thiol aryl azide having the structure;
##STR43## and salts thereof, wherein, R1 to R4, which may be the same or different, are selected from the group consisting of H, alkyl, nitro, hydroxy, halide, carboxylic acid, methoxy, cyano, amino groups;wherein E is a chain of C1 -C20 having chain constituents selected from the group consisting of alkyl, alkenyl, alkynyl and heteroatoms, said chain constituents having substituents selected from the group consisting of H, C1 -C6 alkyl, aryl, ester, ether, amine, amide and halo groups; and wherein Y is a group capable of protecting the sulfur moiety from chemical reaction and removable so as to generate a thiol moiety b) a reagent to remove the protecting group so as to generate a terminal thiol; and c) a thiol-reactive label or ligand capable of reacting with the thiol. - View Dependent Claims (9)
-
Specification