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Methods of analysis and manipulating of DNA utilizing mismatch repair systems

DC
  • US 5,702,894 A
  • Filed: 06/02/1995
  • Issued: 12/30/1997
  • Est. Priority Date: 05/12/1989
  • Status: Expired due to Term
First Claim
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1. A method for removing DNA molecules containing one or more base pair mismatches in a population of DNA duplexes that have been produced by enzymatic amplification, potentially containing one or more base pair mismatches, comprising the steps of:

  • contacting said population of DNA duplexes with a mismatch repair system under conditions such that one or more components of said mismatch repair system form a specific complex with a base pair mismatch contained in a DNA duplex having a base pair mismatch, andremoving said DNA duplex containing said complex or the product of said complex from the population of duplex molecules without the use of an additional agent capable of enzymatic digestion of said DNA duplexes which is not a component of said mismatch repair system, prior to said removal.

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