Magnetic material attracting/releasing control method making use of a pipette device and various types of analyzer using the method

• US 5,702,950 A
• Filed: 06/05/1995
• Issued: 12/30/1997
• Est. Priority Date: 06/15/1994
• Status: Expired due to Term

• Current Assignee: Mortarless Technologies LLC
• Original Assignee: Mortarless Technologies LLC
• Inventors: Tajima, Hideji
• Primary Examiner(s): Le, Long V.
• Application Number:
  US08/462,434
• Time in Patent Office: 939 Days
• Field of Search: 436/43, 436/49, 436/47, 436/54, 436/174, 436/177, 436/180, 436/807, 436/809, 436/810, 422/63, 422/64, 422/65, 422/67, 422/100, 422/101, 210/695, 210/222, 210/223
• US Class Current: 436/49
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical applications
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A magnetic material attracting/releasing control method makes use of a pipette device sucking a liquid from or discharging a liquid into a container. A magnet body or bodies are provided in a liquid suction line of the pipette device, and any magnetic material contained in liquid sucked into the liquid suction line by magnetic force are deposited on the internal surface of the line. The magnetic material is released and discharged together with liquid from the liquid suction line to a state where the effect of the magnetic force generated by the magnet body is cut off.

Claims

1. A magnetic substance attracting/releasing control method comprises the steps of:

providing a pipette device having a liquid suction line including a liquid inlet end for sucking a liquid containing said magnetic substance from a container and discharging said liquid through said liquid inlet end, and a magnet body or magnet bodies being detachably fitted to an external peripheral surface of said liquid suction line of said pipette device;

said pipette device providing attracting/releasing control by absorbing and maintaining said magnetic substance contained in said liquid and attracted to said liquid suction line due to magnetism in said magnet body or bodies on an internal surface of said liquid suction line, said magnetic substance being maintained on said internal surface of said pipette device and also by releasing said magnetic substance from said liquid suction line by means of interrupting effect by magnetism in said magnet body or bodies so that said substance is discharged together with said liquid to outside of said liquid suction line through said liquid inlet end.

2. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein a plurality of said liquid suction lines are provided in parallel to each other and operations of said liquid suction lines for sucking and discharging said liquid are driven and controlled so that said magnetic material is concurrently sucked and discharged at the same time respectively.

3. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein a plurality of said liquid suction lines are provided parallel to to one another, and each of said liquid suction lines is driven and controlled so that a liquid in each liquid suction line is sucked and discharged independently at a different time and through a specific process to each liquid suction line respectively to attract or release magnetic material.

4. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein said liquid suction line and magnetic body or bodies are integrated into a unit and a plurality of said units are provided along a container transfer line.

5. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein at least one unit containing said magnet bodies is provided on said liquid suction line.

6. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein said magnet body or bodies are provided outside said liquid suction line.

7. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein said magnet body or bodies are mounted on said liquid suction line.

8. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein said magnet body comprises a permanent magnet, said magnetic material contained in said liquid and attracted by said liquid suction line is absorbed to and deposited on said internal surface of said liquid suction line by getting said magnet body closer to said liquid suction line, and said magnetic material is discharged together with said liquid to outside said liquid suction line by separating said magnetic material from said liquid suction line by means of getting said magnet body away from said liquid suction line.

9. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein said magnet body comprises an electromagnet, said magnetic material contained in a liquid and attracted by said liquid suction line is absorbed and maintained on said internal surface of said liquid suction line by generating a magnetic force with said electromagnet, and said magnetic material is separated from said liquid suction line and discharged together with a liquid from said liquid suction line when a magnetic force generated by said electromagnet is eliminated or fully reduced.

10. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein a pipette tip is dismountably mounted onto a tip section of said liquid sucking side of said liquid suction line to form said liquid suction line, and a magnetic force generated by said magnet body provides an effect over magnetic material in a liquid stored in said pipette tip.

11. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 10, wherein said pipette tip is transferred to a specific processing position according to the particular inspection method used with magnetic material absorbed and maintained on said internal surface thereof.

12. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 10, wherein said pipette tip is repeatedly used only for a single specimen according to the specified inspection method required by said specimen.

13. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 10, wherein operations for separating said magnetic material from said liquid containing it therein, agitation and cleaning are executed after inside and outside of a liquid-contacting section of said liquid suction line by means of sucking and discharging said liquid repeatedly to a degree at which cross contamination does not occur.

14. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 1, wherein separation of said magnetic material from said liquid containing it therein, agitation, and cleaning operations are executed by conducting said operations for sucking and discharging said liquid with said liquid suction line once or more.

15. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 14, wherein said separation of magnet material from said liquid containing it therein is executed by discharging only said liquid with said magnetic material kept attracted by said magnet body.

16. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 14, wherein said agitation is executed, after a pipette tip has been inserted into a liquid stored in another container with magnetic material attracted on an internal surface of said pipette tip by said magnet body, by sucking and discharging said liquid repeatedly in a state where said operations are not affected by a magnet force generated by said magnet body.

17. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 14, wherein said cleaning operation is executed, after a pipette tip is transferred to a cleaning position with said magnetic material attracted onto an internal surface of said pipette tip by said magnet body, by sucking and discharging a cleaning liquid repeatedly.

18. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 14, wherein said cleaning operation with said cleaning liquid is executed with magnetic material attracted onto an internal surface of said pipette tip.

19. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 18, wherein said cleaning with said cleaning liquid is executed by sucking and discharging said cleaning liquid once or more in a stage where said cleaning operations are not affected by a magnetic force generated by said magnet body.

20. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 14, wherein said separation of magnetic material from a liquid containing it therein, agitation, and cleaning are executed by sucking and discharging a liquid previously stored in a liquid storage section provided in a cartridge with one or more liquid storage sections provided therein.

21. A magnetic material attracting/releasing control method which comprises using a pipette device according to claim 14, wherein said separation of magnetic material from a liquid in which it is contained, agitation, and cleaning are executed by discharging a residual liquid from said container to outside of said container with magnetic material deposited on an internal surface of a pipette tip then pouring a liquid required for a subsequent processing into the same container, and sucking and discharging the poured liquid with said pipette tip.