Microcapsules prepared from crosslinkable polysaccharides, polycations and/or lipids and use therefor
First Claim
1. Microcapsules comprising biologically active material encapsulated in a modified biocompatible material which has been subjected to free radical polymerization under biocompatible conditions in an aqueous media that is free of organic solvents, wherein said material has the formula:
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space="preserve" listing-type="equation">A--Xwherein;
A is selected from the group consisting of polysaccharides, polycations and lipids,X is a moiety containing a carbon-carbon double bond or triple bond capable of free radical polymerization; and
A and X are linked covalently through linkages selected from the group consisting of ester, ether, thioether, disulfide, amide, secondary amines, tertiary amines, direct C--C linkages, sulfate esters, sulfonate esters, phosphate esters, urethanes, and carbonates; and
wherein the largest physical dimension of said microcapsule, including the encapsulated material, does not exceed 1 mm.
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Accused Products
Abstract
The present invention relates to a new form of biocompatible materials (e.g., lipids, polycations, polysaccharides) which are capable of undergoing free radical polymerization, e.g., by using certain sources of light; methods of modifying certain synthetic and naturally occurring biocompatible materials to make polymerizable microcapsules containing biological material coated with said polymerizable materials, composites of said polymerizable materials, methods of making microcapsules and encapsulating biological materials therein, and apparatus for making microcapsules containing biological cells (particularly islets of Langerhans) coated with polymerizable alginate or with a composite thereof (e.g., alginate and PEG). The present invention also relates to drug delivery systems relating to the foregoing, as well as bioadhesives and wound dressings made utilizing the foregoing technology.
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Citations
28 Claims
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1. Microcapsules comprising biologically active material encapsulated in a modified biocompatible material which has been subjected to free radical polymerization under biocompatible conditions in an aqueous media that is free of organic solvents, wherein said material has the formula:
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space="preserve" listing-type="equation">A--Xwherein; A is selected from the group consisting of polysaccharides, polycations and lipids, X is a moiety containing a carbon-carbon double bond or triple bond capable of free radical polymerization; and A and X are linked covalently through linkages selected from the group consisting of ester, ether, thioether, disulfide, amide, secondary amines, tertiary amines, direct C--C linkages, sulfate esters, sulfonate esters, phosphate esters, urethanes, and carbonates; and wherein the largest physical dimension of said microcapsule, including the encapsulated material, does not exceed 1 mm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method of making a microcapsule containing biologically active material encapsulated therein, said method comprising:
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a) suspending said biologically active material in aqueous media that is free of organic solvents, along with a mixture of a modified biocompatible polymerizable material capable of undergoing free radical polymerization and a free radical initiating system, wherein said modified biocompatible material has the formula;
space="preserve" listing-type="equation">A--Xwherein; A is selected from the group consisting of polysaccharides, polycations and lipids, and X is a moiety containing a carbon-carbon double bond or triple bond capable of free radical polymerization; and A and X are linked covalently through linkages selected from the group consisting of ester, ether, thioether, disulfide, amide, secondary amines, tertiary amines, direct C--C linkages, sulfate esters, sulfonate esters, phosphate esters, urethanes, and carbonates; b) forming discrete particles comprising said biologically active material surrounded by the modified biocompatible polymerizable material, wherein the largest physical dimension of said discrete particles, including the biologically active material, does not exceed 1 mm; and c) subjecting the modified biocompatible polymerizable material to free radical generating conditions. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
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28. The method according to claim 21, further comprising subjecting said modified biocompatible polymerizable material to ionic crosslinking conditions.
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Specification