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Systematic evolution of ligands by exponential enrichment: chemi-SELEX

  • US 5,705,337 A
  • Filed: 03/08/1995
  • Issued: 01/06/1998
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for identifying nucleic acid ligands that bind covalently with a target molecule from a candidate mixture of nucleic acids wherein each nucleic acid in said candidate mixture comprises a chemically reactive functional unit, said method comprising:

  • a) preparing a candidate mixture of said nucleic acids;

    b) contacting said candidate mixture with said target molecule, wherein nucleic acids which bind covalently with said target molecule form nucleic acid-target molecule complexes;

    c) partitioning the nucleic acid-target molecule complexes from the remainder of the candidate mixture, whereby nucleic acid ligands that bind covalently with the target molecule are identified.

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