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Method for detecting a target nucleic acid by using an interaction of two kinds of reagents

  • US 5,705,346 A
  • Filed: 06/25/1996
  • Issued: 01/06/1998
  • Est. Priority Date: 11/27/1992
  • Status: Expired due to Term
First Claim
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1. A method for detecting a target nucleic acid which comprises:

  • (a) providing a sample containing a single stranded target nucleic acid having a specific base sequence;

    (b) providing a probe having a base sequence complementary to the specific base sequence;

    (c) forming a double-stranded hybrid between the target nucleic acid and the probe in the presence of two kinds of reagents both of which coexisting in the sample and being capable of interacting with each other through the double-stranded hybrid without direct physical contact between the two kinds of reagents to cause an irreversible detectable change in at least one of the reagents;

    (d) causing repeated formation and dissociation of the double stranded hybrid to generate the interaction between the two kinds of reagents which results in accumulations of irreversibly changed reagent which provides the detectable change; and

    (e) detecting the change provided from the accumulated irreversibly changed reagent to identify the target nucleic acid in the sample.

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