Analytical devices and methods of use
First Claim
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1. An analytical device for use in assay procedures, comprising at least one liquid flow channel of porous material for capillary liquid flow therealong, the channel having:
- a channel end for liquid application thereto,an analytical site, said channel defining a liquid flow path leading in a downstream direction from said channel end to said analytical site,a localized reagent site said channel end and said analytical site, anda permanent liquid impermeable barrier in said channel between said channel end and said analytical site, said barrier being constructed and adapted to create a substantially stagnant zone by intercepting said liquid flow path and extending partially across the channel width, sufficiently close to said localized reagent site that, on application of a reagent to said localized reagent site and application of liquid to said channel end, said substantially stagnant zone is created whereby the distribution of said reagent is elongated as it is delivered from said reagent site to said analytical site by entrainment in said capillary flow.
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Abstract
An analytical device (1) for use in assay procedures comprises at least one liquid flow channel of porous material leading from a channel end to an analytical site (2) via a localized reagent site (3). A liquid-impermeable barrier (6) is arranged adjacent to the reagent site (3) in the liquid flowpath to slow the transport of the reagent to the analytical site (2) by creating a substantially stagnant zone (10) which has the effect of elongating the distribution of reagent in the liquid flow.
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Citations
17 Claims
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1. An analytical device for use in assay procedures, comprising at least one liquid flow channel of porous material for capillary liquid flow therealong, the channel having:
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a channel end for liquid application thereto, an analytical site, said channel defining a liquid flow path leading in a downstream direction from said channel end to said analytical site, a localized reagent site said channel end and said analytical site, and a permanent liquid impermeable barrier in said channel between said channel end and said analytical site, said barrier being constructed and adapted to create a substantially stagnant zone by intercepting said liquid flow path and extending partially across the channel width, sufficiently close to said localized reagent site that, on application of a reagent to said localized reagent site and application of liquid to said channel end, said substantially stagnant zone is created whereby the distribution of said reagent is elongated as it is delivered from said reagent site to said analytical site by entrainment in said capillary flow. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of controlling the rate of delivery of a reagent to an analytical site in an assay procedure, said method comprising:
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providing at least one porous liquid flow channel in which an analytical site is located, said channel also having a channel end for liquid application thereto, and incorporating a localized reagent site, said channel also having a permanent liquid impermeable barrier between the channel end and said analytical site, said barrier extending partially across the width of said channel; providing a selected reagent at the reagent site; introducing liquid to said channel end to initiate capillary liquid flow along the flow channel to the analytical site via the reagent site, the impermeable barrier creating a substantially stagnant zone, said impermeable barrier being sufficiently close to the reagent site such that the substantially stagnant zone results in a slowing of transport of the reagent relative to transport of said liquid as it is delivered to the analytical site by entrainment in said capillary flow. - View Dependent Claims (15, 16)
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17. An analytical device for use in assay procedures, comprising at least one liquid flow channel of porous material for capillary liquid flow therealong, the channel having:
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a channel end for liquid application thereto, an analytical site, the channel defining a liquid flow path leading in a downstream direction from said channel end to said analytical site, a localized reagent site in said liquid flow path between said channel end and said analytical site, and permanent liquid impermeable barrier means between said channel end and said analytical site and extending partially across the channel width for intercepting said liquid flow path on application of a reagent to said localized reagent site and application of liquid to said channel end, said barrier means being constructed and adapted to create a substantially stagnant zone, whereby transport of said reagent from said reagent site to said analytical site is slowed relative to transport of said liquid.
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Specification