Apparatus and methodology for determining oxygen tension in biological systems
First Claim
1. A method for determining oxygen tension in a biological system, comprising the steps of introducing a physiologically acceptable paramagnetic material to a surface of the biological system, said paramagnetic material having electron paramagnetic resonance spectra responsive to the presence of oxygen, protecting the material from sources of oxygen that are outside of the biological system, and determining the oxygen tension from the electron paramagnetic resonance spectra of the material.
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Abstract
The invention provides apparatus and methods for measuring oxygen tensions in biological systems utilizing physiologically acceptable paramagnetic material, such as India ink or carbon black, and electron paramagnetic resonance (EPR) oximetry. India ink is introduced to the biological system and exposed to a magnetic field and an electromagnetic field in the 1-2 GHz range. The EPR spectra is then measured at the biological system to determine oxygen concentration. The EPR spectra is determined by an EPR spectrometer that adjusts the resonator to a single resonator frequency to compensate for movements of the biological system, such as a human or animal. The biological system can also include other in vivo tissues, cells, and cell cultures to directly measure pO2 non-destructively. The paramagnetic material can be used non-invasively or invasively depending on the goals of the pO2 measurement. A detecting inductive element, as part of the EPR spectrometer resonator, is adapted relative to the measurement particularities.
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Citations
11 Claims
- 1. A method for determining oxygen tension in a biological system, comprising the steps of introducing a physiologically acceptable paramagnetic material to a surface of the biological system, said paramagnetic material having electron paramagnetic resonance spectra responsive to the presence of oxygen, protecting the material from sources of oxygen that are outside of the biological system, and determining the oxygen tension from the electron paramagnetic resonance spectra of the material.
- 8. A method for determining oxygen tension in a biological system, comprising the steps of enclosing a paramagnetic material within an inert carrier, said paramagnetic material having electron paramagnetic resonance spectra responsive to the presence of oxygen, introducing the carrier to within the biological system, and determining the oxygen tension of the biological system from the electron paramagnetic resonance spectra of the material within the carrier, wherein the material is disposed within the carrier during the step of determining.
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