Spectroscopic method for quantitatively determining the change of concentration of a light or other radiation absorbing compound in a medium which is interrogated through an intervening medium

• US 5,706,821 A
• Filed: 12/30/1994
• Issued: 01/13/1998
• Est. Priority Date: 12/30/1994
• Status: Expired due to Fees

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK
• Inventors: Cope, Mark, Matcher, Stephen John, Delpy, David Thomas
• Primary Examiner(s): Casler, Brian L.
• Application Number:
  US08/366,588
• Time in Patent Office: 1,110 Days
• Field of Search: 128/664, 128/665, 128/633, 356/433, 356/39, 356/40, 356/341, 356/342, 356/239, 250/330, 250/339.11, 250/574, 250/358.1
• US Class Current: 600/310
• CPC Class Codes: A61B 2562/0242 for varying or adjusting the optical path length in the tissue
• CPC Class Codes: A61B 5/14553 specially adapted for cerebral tissue
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

A spectrometric technique is described for quantitatively determining the change in concentration of a substance which is contained in a medium which both absorbs and scatters spectroscopically interrogated radiation, and in particular to the specific case in which the change in concentration of the substance occurs in one spatially distinct region of the medium. The medium includes a plurality of such regions displaying potentially different absorption and scattering properties to the radiation. Specific applications are disclosed involving the noninvasive determination of changes in concentration of the substances oxyhemoglobin and deoxyhemoglobin and/or changes in the redox state of the substance cytochrome oxidase within the brain or skeletal muscle, and in cases where the brain or skeletal muscle is interrogated by radiation in the near-infrared part of the electromagnetic spectrum through intervening surface tissues, i.e., cortical bone or adipose tissue, where it may be assumed that no changes of concentration of said substances occur within said surface tissues.

Claims

1. A method of determining a change in concentration of at least one subject substance in one region of a plurality of regions of a medium, the one region being adaptable to both absorb and scatter radiation, substantially all of the change in concentration of the at least one subject substance in the medium occurring in the one region of the plurality of regions, said method comprising:

introducing into and propagating through the medium first interrogating radiation which is penetrative of the medium;

detecting the first interrogating radiation which has propagated through the medium and determining a total differential pathlength traversed by the first interrogating radiation in the medium;

identifying a plurality of reference substances, each of which possesses a sufficiently distinct specific absorption coefficient to interrogating radiation and a known concentration in each region of the plurality of regions of the medium, the concentration of each of the plurality of reference substances in the one region being different from the concentration of the same reference substance in each of the other regions of the plurality of regions of the medium;

for each reference substance of the plurality of reference substances, introducing into and propagating through the medium second interrogating radiation which is penetrative of the medium, detecting and spectrometrically analyzing the second interrogating radiation which has propagated through the medium, and determining an intensity of a spectral absorption feature of the reference substance;

determining a partial differential pathlength of the one region by simultaneously solving a first linearly independent equation for each reference substance and a second equation, the first and second equations respectively being expressed as follows;

##EQU5## where a_i is the intensity of the spectral absorption feature of the reference substance;

C_ij is the known concentration of the reference substance in the region j of the plurality of regions;

.di-elect cons._i is the absorption coefficient of the reference substance;

Z_T is the total differential pathlength;

Z_j is a partial differential pathlength of the radiation in the region j of the plurality of regions; and

n is a total number of regions in the plurality of regions;

introducing into and propagating through the medium third interrogating radiation which is penetrative of the medium;

detecting and spectrometrically analyzing the third interrogating radiation which has propagated through the medium and determining a change in an intensity of a spectral feature of the at least one subject substance; and

obtaining the change in concentration of the at least one subject substance in the one region of the plurality of regions of the medium by solving a third equation, the third equation being expressed as follows;

space="preserve" listing-type="equation">C=A/(.di-elect cons.·

Z)

where

C is the change in concentration of the at least one subject substance in the one region of the plurality of regions of the medium

A is the change in the intensity of the spectral feature of the at least one subject substance;

.di-elect cons. is an absorption coefficient of the at least subject substance; and

Z is the partial differential pathlength of the one region.

2. A method according to claim 1, wherein said step of determining the total differential pathlength is performed by a time-of-flight or phase delay technique.

3. A method according to claim 1, wherein the second interrogating radiation is electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human head and an animal head, wherein the plurality of regions comprises a brain region and a skull region, wherein the plurality of reference substances includes tissue water, and wherein said step of obtaining the change in concentration of the at least one subject substance comprises determining changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the brain.

4. A method according to claim 1, wherein the second interrogating radiation is electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human limb and an animal limb, wherein the plurality of regions comprises cortical bone, skeletal muscle and surface adipose tissue, wherein the plurality of reference substances are tissue water and lipid, and wherein said step of obtaining the change in concentration of the at least one subject substance comprises determining changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the skeletal muscle.

5. A method according to claim 1, wherein the second interrogating radiation is electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human limb and an animal limb, wherein the plurality of regions comprises cortical bone, skeletal muscle and surface adipose tissue, wherein the plurality of reference substances are tissue water, lipid and deoxyhemoglobin, and wherein said step of obtaining the change in concentration of the at least one subject substance comprises determining changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the skeletal muscle, said method further comprising determining a concentration of deoxyhemoglobin in the skeletal muscle by blood samples, biopsy samples, or blood and biopsy samples, and setting a concentration of deoxyhemoglobin in the adipose tissue and the cortical bone as zero.

6. A method according to claim 1, wherein one or more of the plurality of reference substances are introduced from the external environment into the medium.

7. A method according to claim 6, further comprising establishing the concentrations of the introduced reference substances by exploiting differential accumulation rates within the regions containing the introduced reference substances.

8. A method according to claim 6, further comprising establishing the concentrations of the introduced reference substances by exploiting differential transit times of the introduced reference substances between a spatial location of introduction of the introduced reference substances and spatial locations of the regions of the medium.

9. A method according to claim 6, wherein the introduced reference substances are indocyanine-green, saline solution, and deuterium-oxide.

10. A method of determining a change in concentration of at least one subject substance in one region of a plurality of regions of a medium, the one region being adaptable to both absorb and scatter radiation, substantially all of the change in concentration of the at least one subject substance in the medium occurring in the one region of the plurality of regions, said method comprising:

introducing into and propagating through the medium first interrogating radiation which is penetrative of the medium;

detecting the first interrogating radiation which has propagated through the medium and determining a total differential pathlength traversed by the first interrogating radiation in the medium;

identifying at least one reference substance, the at least one reference substance possessing a sufficiently distinct specific absorption coefficient to the first interrogating radiation and a known concentration in each region of the plurality of regions of the medium;

introducing into and propagating through the medium second interrogating radiation which is penetrative of the medium, detecting and spectrometrically analyzing the second interrogating radiation which has propagated through the medium, and determining an intensity of a spectral feature of the at least one reference substance;

introducing into and propagating through the medium third interrogating radiation which is penetrative of the medium;

detecting and spectrometrically analyzing the third interrogating radiation which has propagated through the medium and determining a change in an intensity of a spectral feature of the at least one subject substance;

determining a partial differential pathlength of the one region by simultaneously solving first and second equations, the first and second equations respectively being expressed as follows;

##EQU6## where a_ref is the intensity of the spectral feature of the at least one reference substance;

C_j is the known concentration of the at least one reference substance in region j of the plurality of regions;

.di-elect cons._ref is the absorption coefficient of the at least one reference substance;

Z_j is a partial differential pathlength in the region j; and

Z_T is the total differential pathlength;

n is a total number of regions in the plurality of regions; and

obtaining the change in concentration of the at least one subject substance in the one region of the plurality of regions of the medium by solving a third equation, the third equation being expressed as follows;

space="preserve" listing-type="equation">C=A/(.di-elect cons.·

Z)

where

A is the change in the intensity of the spectral feature of the at least one subject substance;

C is the change in concentration of the at least one subject substance in the one region of the plurality of regions of the medium;

.di-elect cons. is an absorption coefficient of the at least subject substance; and

Z is the partial differential pathlength of the one region.

11. A method according to claim 10, wherein said step of determining the total differential pathlength traversed by the first interrogating radiation in the medium comprises measuring a time required for the first interrogating radiation to propagate from a point of entry into the medium to a point of exit from the medium, and multiplying the measured time by the speed of light.

12. A method according to claim 10, wherein said step of determining the total differential pathlength traversed by the first interrogating radiation in the medium comprises measuring a phase delay induced in the first interrogating radiation which propagates through the medium, and determining a phase shift and multiplying the phase shift by a wavelength of a sinusoidal intensity modulation within the medium.

13. A method according to claim 10, wherein the second interrogating radiation is electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human head and an animal head, wherein the plurality of regions comprises a brain region and a skull region, wherein the at least one reference substance is tissue water, and wherein said step of obtaining the change in concentration of the at least one subject substance comprises determining changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the brain.

14. A method according to claim 10, wherein the second interrogating radiation is electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human limb and an animal limb, wherein the plurality of regions comprises cortical bone, skeletal muscle and surface adipose tissue, wherein the at least one reference substance comprises tissue water and lipid, and wherein said step of obtaining the change in concentration of the at least one subject substance comprises determining changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the skeletal muscle.

15. A method according to claim 10, wherein the second interrogating radiation is electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human limb and an animal limb, wherein the plurality of regions comprises cortical bone, skeletal muscle and surface adipose tissue, wherein the at least one reference substance comprises tissue water, lipid and deoxyhemoglobin, and wherein said step of obtaining the change in concentration of the at least one subject substance comprises determining changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the skeletal muscle, said method further comprising determining a concentration of deoxyhemoglobin in the skeletal muscle by blood samples, biopsy samples, or blood and biopsy samples, and setting a concentration of deoxyhemoglobin in the adipose tissue and the cortical bone as zero.

16. A method according to claim 10, wherein the at least one reference substance includes a plurality of reference substances, and one or more of which are introduced from the external environment into the medium.

17. A method according to claim 16, further comprising establishing the concentrations of the introduced reference substances by exploiting differential accumulation rates within the regions containing the introduced reference substances.

18. A method according to claim 16, further comprising establishing the concentrations of the introduced reference substances by exploiting differential transit times of the introduced reference substances between a spatial location of introduction of the introduced reference substances and spatial locations of the regions of the medium.

19. A method according to claim 16, wherein the introduced reference substances are indocyanine-green, saline solution, and deuterium-oxide.

20. An apparatus for determining a change in concentration of at least one subject substance in one region of a plurality of regions of a medium, the one region being adaptable to both absorb and scatter radiation, substantially all of the change in concentration of the at least one subject substance in the medium occurring in the one region of the plurality of regions, the plurality of regions each comprising at least one reference substance possessing a distinct specific absorption coefficient to a specific radiation and a known concentration, said apparatus comprising:

a first light source for introducing into and propagating through the medium first interrogating radiation which is penetrative of the medium;

a first photodetecting unit for detecting the first interrogating radiation which has propagated through the medium to measure an intensity of a spectral feature of the at least one subject substance or the at least one reference substance;

a second light source for introducing into and propagating through the medium second interrogating radiation which is penetrative of the medium;

a second photodetecting unit for detecting the second interrogating radiation which has propagated through the medium to measure a total differential pathlength traversed by the second interrogating radiation in the medium;

a first processing unit for receiving the intensity of the spectral feature of the at least one subject substance or at least one reference substance from said first photodetecting unit, for receiving the total differential pathlength from said second photodetecting unit, and for determining the partial differential pathlength of the one region by solving first and second equations, the first and second equations being expressed as follows;

##EQU7## where a_ref is said intensity of said spectral feature of the at least one reference substance;

C_j is the known concentration of the at least one reference substance in region j of the plurality of regions;

.di-elect cons._ref is the absorption coefficient of said at least one reference substance;

Z_j is a partial differential pathlength in the region j; and

Z_T is the total differential pathlength;

n is a total number of regions in the plurality of regions; and

a second processing unit for determining the change in concentration of the at least one substance in the one region of the plurality of regions of the medium by a third equation, the third equation being expressed as follows;

space="preserve" listing-type="equation">C=A/(.di-elect cons.·

Z)

where

A is the change in the intensity of the spectral feature of the at least one subject substance;

C is the change in concentration of the at least one subject substance in the one region of the plurality of regions of the medium;

.di-elect cons. is an absorption coefficient of the at least subject substance; and

Z is the partial differential pathlength of the one region.

21. An apparatus according to claim 20, further comprising a display unit for receiving the change in concentration of the at least one subject substance from said second processing unit and for displaying the change in concentration of the at least one subject substance, said display unit being digital or analog.

22. An apparatus according to claim 21, wherein said display unit is a CRT.

23. An apparatus according to claim 20, wherein said second photodetecting unit is constructed and arranged to measure a time required for the first interrogating radiation to propagate from a point of entry into the medium to a point of exit from the medium and to obtain the total differential pathlength traversed by the interrogating radiation in the medium by multiplying the measured time by the speed of light.

24. An apparatus according to claim 23, wherein said second photodetecting unit is a streak camera.

25. An apparatus according to claim 23, wherein said second light source is a laser light source.

26. An apparatus according to claim 20, wherein said second photodetecting unit is constructed and arranged to measure a phase delay induced in the second interrogating radiation which propagates through the medium and to obtain the total differential pathlength traversed by the second interrogating radiation in the medium by multiplying a measured phased shift by a wavelength of a sinusoidal intensity modulation within the medium.

27. An apparatus according to claim 26, wherein both said second light source and said second photodetecting unit comprise an intensity modulated optical spectrometer.

28. An apparatus according to claim 20, wherein both said first light source and said first photodetecting unit comprise a CCD spectroscopy system.

29. An apparatus according to claim 20, wherein said first light source is a DC lamp.

30. An apparatus according to claim 20, wherein said first photodetecting unit is a spectroscope.

31. An apparatus according to claim 20, wherein said first light source produces electromagnetic radiation in the near-frared part of the spectrum, wherein the medium is selected from the group consisting of a human head an an animal head, wherein the plurality of regions comprises a brain region and a skull region, wherein the at least one reference substance is tissue water, and wherein said second processing unit is adapted to determine changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the brain.

32. An apparatus according to claim 20, wherein the first light source produces electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human limb and an animal limb, wherein the plurality of regions comprises cortical bone, skeletal muscle and surface adipose tissue, wherein the at least one reference substance comprises tissue water and lipid, and wherein said second processing unit is adapted to determine changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the skeletal muscle.

33. An apparatus according to claim 20, wherein the first light source produces electromagnetic radiation in the near-infrared part of the spectrum, wherein the medium is selected from the group consisting of a human limb and an animal limb, wherein the plurality of regions comprises cortical bone, skeletal muscle and surface adipose tissue, wherein the at least one reference substance comprises tissue water, lipid and deoxyhemoglobin, wherein said second processing unit is adapted to determine changes in concentration of oxyhemoglobin and deoxyhemoglobin and changes in redox state of cytochrome oxidase within the skeletal muscle, and wherein said apparatus is adapted to determine a concentration of deoxyhemoglobin in the skeletal muscle by blood samples, biopsy samples, or blood and biopsy samples, and set a concentration of deoxyhemoglobin in the adipose tissue and the cortical bone as zero.