Channel plate for DNA sequencing

• US 5,707,506 A
• Filed: 05/06/1996
• Issued: 01/13/1998
• Est. Priority Date: 10/28/1994
• Status: Expired due to Fees

• Current Assignee: Battelle Memorial Institute
• Original Assignee: Battelle Memorial Institute
• Inventors: Douthart, Richard J., Crowell, Shannon L.
• Primary Examiner(s): Gorgos, Kathryn L.
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US08/643,820
• Time in Patent Office: 617 Days
• Field of Search: 204/606, 204/607, 204/608, 204/609, 204/610, 204/612, 204/613, 204/614, 204/615, 204/616, 204/617, 204/618, 204/619, 204/620, 204/621, 204/470, 204/469, 204/468, 204/467, 204/466, 204/465, 204/464, 204/463, 204/462, 204/461, 204/459, 204/458, 204/457, 204/456
• US Class Current: 204/622
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/4473 by electric means

Abstract

This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface.

Claims

1. A channel plate for electrophoresis sequencing of a DNA sample, said channel plate having (a) a length, a width and a thickness, and further having, (b) a plurality of channels that are parallel placed upon an upper surface of the channel plate, each channel having a depth partially through the thickness and filled with a gel, wherein the improvement comprises:

(c) said channel plate is made from electrically non-conductive, non-cellulosic material, and

(d) a temperature control plate attached to a lower surface wherein a temperature of the channel plate is controlled.

2. The channel plate as recited in claim 1, wherein said temperature control plate contains at least one baffle chamber.

3. The channel plate in claim 1, wherein the channel plate is vertically oriented.

4. The channel plate as recited in claim 1, wherein the channel plate is horizontally oriented.

5. The channel plate as recited in claim 1, wherein said electrically non-conductive, non-cellulosic material is a glass ceramic.

6. The channel plate as recited in claim 5, wherein said glass ceramic is a machinable glass ceramic.

7. A method of preparing a DNA sample for electrophoretic separation, comprising the steps of:

(a) providing a glass ceramic channel plate having a length, width and thickness, further having a plurality of parallel channels formed partially through the thickness and each channel having a first end at a position distant from a first edge, each of the channels extending therefrom to an interface edge;

(b) filling the channels with a gel;

(c) placing a cover plate over the channels thereby covering the channels and confining the gel within the channels;

(d) inserting a comb into the channels;

(e) permitting the gel to polymerize with the comb in place;

(f) withdrawing the comb after polymerization of the gel and leaving, sample wells; and

(g) filling the sample wells with DNA sample.

8. The method as recited in claim 7, wherein said comb is inserted at the first end of the channels.

9. The method as recited in claim 7, wherein said comb is inserted at a top edge.

10. The method as recited in claim 7, further comprising the step of:

degassing the gel prior to filling the channels.

11. The method as recited in claim 7, further comprising attaching a temperature control plate to a lower surface of said channel plate and controlling a temperature of said channel plate.

12. A channel plate for electrophoresis sequencing of a DNA sample, said channel plate having (a) a length, a width and a thickness, and further having, (b) a plurality of channels that are parallel placed upon an upper surface of the channel plate, each channel having a depth partially through the thickness and filled with a gel, wherein the improvement comprises:

(c) said channel plate is made from a glass ceramic.

13. The channel plate in claim 12, further comprising a cover plate for containing the gel.

14. The channel plate as recited in claim 13 wherein said cover plate is made from glass.

15. The channel plate as recited in claim 13, further comprising a temperature control plate attached to a lower surface wherein a temperature of the channel plate is controlled by a fluid flow through the temperature control plate.

16. A method of preparing a DNA sample for electrophoretic separation, comprising the steps of:

(a) providing an electrically non-conducting, non-cellulosic channel plate having a length, width and thickness, further having a plurality of parallel channels formed partially through the thickness and each channel having a first end at a position distant from a first edge, each of the channels extending therefrom to an interface edge;

(b) filling the channels with a gel;

(c) placing a cover plate over the channels thereby covering the channels and confining the gel within the channels;

(d) inserting a comb into the channels;

(e) permitting the gel to polymerize with the comb in place;

(f) withdrawing the comb after polymerization of the gel and leaving the sample wells;

(g) filling the sample wells with DNA sample; and

(h) attaching a temperature control plate to a lower surface of said channel plate for controlling a temperature of said channel plate during the electrophoretic separation.

17. The method as recited in claim 16, wherein said non-electrically conducting, non-cellulosic channel plate is glass ceramic.

18. The method as recited in claim 17, wherein said glass ceramic is machinable glass ceramic.