Mediators to oxidoreductase enzymes
First Claim
1. A process of mediating electron transfer between an electrode and an oxidoreductase enzyme or other electron transfer redox protein using a mediator compound, wherein said mediator compound is a half-sandwich complex of a transition metal, said complex having only a single π
- -ligand with delocalised π
bonding and having the general formula;
space="preserve" listing-type="equation">(π
-ligand)M(not-π
-ligand).sub.nwherein the complex may be charged or neutral, π
-ligand represents the single π
-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not π
-ligands, and the n not-π
-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M.
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Abstract
Half-sandwich complexes of transition metals having a single π-ligand with delocalised π bonding are of use in mediating electron transfer between an electrode and a redox protein, for example an oxidoreductase enzyme such as in an amperometric enzyme sensor. Suitable compounds include complexes of the general formula:
(π-ligand)M(not-π-ligand).sub.n
wherein the complex is charged or neutral, π-ligand represents the single π-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not π-ligands, and the n not-π-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transitional metal M.
362 Citations
36 Claims
-
1. A process of mediating electron transfer between an electrode and an oxidoreductase enzyme or other electron transfer redox protein using a mediator compound, wherein said mediator compound is a half-sandwich complex of a transition metal, said complex having only a single π
- -ligand with delocalised π
bonding and having the general formula;
space="preserve" listing-type="equation">(π
-ligand)M(not-π
-ligand).sub.nwherein the complex may be charged or neutral, π
-ligand represents the single π
-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not π
-ligands, and the n not-π
-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M. - View Dependent Claims (2, 3, 4, 5, 6, 28, 29, 30)
- -ligand with delocalised π
-
7. An amperometric enzyme sensor for an analyte, which sensor employs a transition metal half-sandwich compound as the mediator, said half-sandwich transition metal half-sandwich compound including a transition metal complexed to only a single π
- ligand with delocalized π
bonding and having the general formula;
space="preserve" listing-type="equation">(π
-ligand)M(not-π
-ligand).sub.nwherein the complex may be charged or neutral, π
-ligand represents the single-π
-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not π
ligands, and the n not-π
-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M. - View Dependent Claims (10, 11, 12, 13, 14, 31, 32, 33)
- ligand with delocalized π
-
8. A disposable dry strip test element for an amperometric enzyme sensor, said disposable test element carrying a working electrode, test reagents comprising at least an oxidoreductase enzyme and a half-sandwich complex of a transition metal as the mediator for generation of a current indicative of the level of analyte, and a reference electrode, said complex having a single π
- ligand with delocalized π
bonding and having the general formula;
space="preserve" listing-type="equation">(π
-ligand)M(not-π
-ligand).sub.nwherein the complex may be charged or neutral, π
-ligand represents the single-π
-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not π
ligands, and the n not-π
-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transition metal M. - View Dependent Claims (15, 16, 17, 18, 19, 34, 35, 36)
- ligand with delocalized π
-
9. An electrically conductive printing ink which includes a half-sandwich complex of a transition metal as the mediator, said half-sandwich complex having a single π
- ligand with delocalized π
bonding and having the general formula;
space="preserve" listing-type="equation">(π
-ligand)M(not-π
-ligand).sub.nwherein the complex may be charged or neutral, π
-ligand represents the single-π
-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not π
ligands, and the n not-π
-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transitional metal M. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27)
- ligand with delocalized π
Specification