Large area submucosal tissue graft constructs

• US 5,711,969 A
• Filed: 04/07/1995
• Issued: 01/27/1998
• Est. Priority Date: 04/07/1995
• Status: Expired due to Term

• Current Assignee: Clarian Health Partners, Inc.
• Original Assignee: Methodist Hospitals of Dallas, Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Inventors: Patel, Umesh H., Hiles, Michael C., Badylak, Stephen F., Whitson, Bryan, Cheng, Boyle, Kokini, Klod
• Primary Examiner(s): Witz, Jean C.
• Application Number:
  US08/418,515
• Time in Patent Office: 1,026 Days
• Field of Search: 424/551, 623/11, 623/13
• US Class Current: 424/551
• CPC Class Codes: A61F 2/0063 Implantable repair or support meshes, e.g. hernia meshes
• CPC Class Codes: A61K 35/38 Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
• CPC Class Codes: A61L 27/3629 Intestinal tissue, e.g. small intestinal submucosa
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

A unitary heterolaminar tissue graft construct is prepared by fusing partially overlapped strips or sheets of submucosa tissue. The submucosa components are fused by compressing at least the overlapped positions of said strips between two surfaces under conditions that allow or promote dehydration of the compressed submucosa sheets. Three dimensional graft constructs can be prepared by using complementary non-planar compressive surfaces.

Claims

1. A unitary heterolaminar tissue graft construct consisting essentially of multiple strips of intestinal submucosa tissue fused to one another by compressing the strips under dehydrating conditions, said heterolaminar construct having a variable number of layers of intestinal submucosa tissue superimposed at different points on the construct and the construct has a surface area greater than any one of the individual strips used to form said construct.

3. The graft construct of claim 1, wherein the intestinal submucosa tissue comprises the tunica submucosa delaminated from both the tunica muscularis and the luminal portion of the tunica mucosa.

4. The graft construct of claim 1, wherein the submucosa strips are conditioned to have a strain of no more than 20%.

2. A unitary tissue graft construct consisting essentially of multiple strips of intestinal submucosa tissue fused to one another by compressing the strips under dehydrating conditions, said graft construct formed to have a three dimensional shape and a variable number of layers of submucosal tissue superimposed at different points on the graft construct, and wherein said graft construct has a surface area greater than any one of the individual strips used to form said graft construct.

5. A unitary heterolaminar tissue graft construct consisting essentially of multiple strips of intestinal submucosa tissue fused to one another by compressing the strips under dehydrating conditions, said heterolaminar construct having only a single layer of intestinal submucosa tissue at different points on the construct, and wherein the construct has a surface area greater than any one of the individual strips used to form said construct.