Papillomavirus probe and process for in vitro diagnosis of papillomavirus infections
First Claim
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1. Isolated DNA of a human papillomavirus (HPV), wherein said DNA is about 7,000 to about 8,000 base pairs;
- andsaid DNA is obtained from a virus selected from the group consisting of HPV-2d, HPV-10b, HPV-14a, HPV-14b, HPV-15, HPV-17a, HPV-17b, HPV-19, HPV-20, HPV-21, HPV-22, HPV-23, HPV-24, HPV-28, HPV-29, HPV-31, HPV-32, HPV-IP2, and HPV-IP4.
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Abstract
The invention relates to human papillomaviruses HPV, particularly to HPV-DNAs isolated from papillomaviruses HPV-2d, HPV-10b, HPV-14a, HPV-14b, HPV-15, HPV-17a, HPV-17b, HPV-19, HPV-20, HPV-21, HPV-22, HPV-23, HPV-24, HPV-28, HPV-29, HPV-31, HPV-32, HPV-IP2 and HPV-IP4. The invention also relates to DNA capable of hybridizing with the HPV-DNAs or fragments thereof, to kits containing distinct groups of probes containing one or more of these HPV-DNAs or fragments thereof, and to procedures for detecting and identifying HPV in tissue.
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Citations
17 Claims
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1. Isolated DNA of a human papillomavirus (HPV), wherein said DNA is about 7,000 to about 8,000 base pairs;
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said DNA is obtained from a virus selected from the group consisting of HPV-2d, HPV-10b, HPV-14a, HPV-14b, HPV-15, HPV-17a, HPV-17b, HPV-19, HPV-20, HPV-21, HPV-22, HPV-23, HPV-24, HPV-28, HPV-29, HPV-31, HPV-32, HPV-IP2, and HPV-IP4. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A diagnostic kit comprising one or more human papillomavirus (HPV) probes, wherein said kit contains the following groups of probes:
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(1) the HPV DNA of HPV-2d; (2) at least one of the HPV DNAs of HPV-10b, HPV-28, and HPV-29; (3) at least one of the HPV DNAs of HPV-17 and HPV-24; (4) at least one of the HPV DNAs of HPV-14, HPV-15, HPV-17, HPV-19, HPV-20, HPV-21, HPV-22, and HPV-23; (5) at least one of the HPV DNAs of HPV-15 and HPV-17; (6) the HPV DNA of HPV-24; (7) at least one of the HPV DNAs of HPV-14 and HPV-32; (8) the HPV DNA of HPV-31; (9) the HPV DNA of HPV-32; wherein the human papillomavirus (HPV) DNA of the groups is chosen such that if each of the nine groups contains only one HPV DNA group member, the HPV DNA of each of the nine groups will be different from each other.
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9. A diagnostic kit comprising one or more human papillomavirus (HPV) probes, wherein said kit contains the following groups of probes:
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(1) the HPV DNA of HPV-2d; (2) at least one of the HPV DNAs of HPV-10b, HPV-28, and HPV-29; (3) at least one of the HPV DNAs of HPV-17 and HPV-24; (4) at least one of the HPV DNAs of HPV-14, HPV-15, HPV-17, HPV-19, HPV-20, HPV-21, HPV-22, and HPV-23; (5) at least one of the HPV DNAs of HPV-15 and HPV-17; (6) the HPV DNA of HPV-24; (7) at least one of the HPV DNAs of HPV-14 and HPV-32; (8) the HPV DNA of HPV-31; (9) the HPV DNA of HPV-32; (10) at least one of the HPV DNAs of HPV-16, HPV-18, and HPV-IP2; wherein the HPV DNA of the groups is chosen such that if each of the ten groups contains only one HPV DNA group member, the HPV-DNA of each of the ten groups will be different from each other.
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10. An isolated DNA, wherein said DNA is selected from the group consisting of
(a) HPV-2d DNA between the restriction sites shown in FIG. 1; -
(b) HPV-10b DNA between the restriction sites shown in FIG. 2; (c) HPV-14a DNA between the restriction sites shown in FIG. 4a; (d) HPV-14b DNA between the restriction sites shown in FIG. 4a; (e) HPV-15 DNA between the restriction sites shown in FIG. 5; (f) HPV-17a DNA between the restriction sites shown in FIG. 5; (g) HPV-17b DNA between the restriction sites shown in FIG. 5; (h) HPV-19 DNA between the restriction sites shown in FIG. 4b; (i) HPV-20 DNA between the restriction sites shown in FIG. 4b; (j) HPV-21 DNA between the restriction sites shown in FIG. 4b; (k) HPV-22 DNA between the restriction sites shown in FIG. 4b; (l) HPV-23 DNA between the restriction sites shown in FIG. 4b; (m) HPV-24 DNA between the restriction sites shown in FIG. 6; (n) HPV-28 DNA between the restriction sites shown in FIG. 2; (o) HPV-29 DNA between the restriction sites shown in FIG. 2; (p) HPV-31 DNA between the restriction sites shown in FIG. 8; (q) HPV-32 DNA between the restriction sites shown in FIG. 7; (r) HPV-IP2 DNA between the restriction sites shown in FIG. 9; and (s) HPV-IP4 DNA between the restriction sites shown in FIG. 9, and DNA complementary to any one of said DNAs.
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11. The plasmid pBR322/HPV2d.
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12. Human papillomavirus DNA contained in C.N.C.M. (Collection Nationale de Culturae de Microorganisms) Accession No. I-379.
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13. A process for producing a human papillomavirus (HPV) DNA probe, wherein said probe comprises DNA of a human papillomavirus (HPV), wherein
(1) said DNA is about 7,000 to about 8,000 base-pairs; (2) said DNA is obtained from the group of papillomaviruses consisting of HPV-2d, HPV-10b, HPV-14a, HPV-14b, HPV-15, HPV-17a, HPV-17b, HPV-19, HPV-20, HPV-21, HPV-22, HPV-23, HPV-24, HPV-28, HPV-29, HPV-31, HPV-32, HPV-IP2, and HPV-IP4, said process comprising (A) producing a recombinant vector comprising said DNA of a HPV and a suitable vector, wherein said HPV DNA is inserted into said vector at a site permitting the subsequent cloning of said recombinant vector into a suitable host; (B) cloning said recombinant vector into a suitable host; and (C) recovering the resultant cloned recombinant HPV DNA. - View Dependent Claims (14, 15, 16, 17)
Specification