Intraperative, intravascular and endoscopic tumor and lesion detection and therapy with monovalent antibody fragments
First Claim
Patent Images
1. A method for close-range tumor detection and, optionally, treatment, during an operative, intravascular or endoscopic procedure, wherein the method comprises the steps of:
- injecting a patient subject to such a procedure parenterally with an effective amount of a labeled monovalent antibody fragment or subfragment which specifically binds antigen produced by or associated with a tumor;
conducting the procedure within 48 hours of the injection;
scanning the accessed interior of the patient at close range with a detection means for detecting the presence of the labeled antibody fragment or subfragment;
locating the sites of accretion of the labeled antibody fragment or subfragment by detecting elevated levels of the labeled antibody fragment or subfragment at such sites with the detection means, without the use of a clearing agent, contrast agent or subtraction agent, and optionally, treating the detected sites of accretion of said labeled antibody fragment or subfragment.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods are provided for close-range intraoperative, endoscopic and intravascular detection and treatment of lesions, including tumors and non-malignant lesions. The methods use agents labeled with isotopic and non-isotopic agents. Also provided are methods for detection and treatment of lesions with photodynamic agents and methods of treating lesions with a protein conjugated to an agent capable of being activated to emit Anger electron or other ionizing radiation. Compositions and kits useful in the above methods are also provided.
270 Citations
52 Claims
-
1. A method for close-range tumor detection and, optionally, treatment, during an operative, intravascular or endoscopic procedure, wherein the method comprises the steps of:
-
injecting a patient subject to such a procedure parenterally with an effective amount of a labeled monovalent antibody fragment or subfragment which specifically binds antigen produced by or associated with a tumor; conducting the procedure within 48 hours of the injection; scanning the accessed interior of the patient at close range with a detection means for detecting the presence of the labeled antibody fragment or subfragment; locating the sites of accretion of the labeled antibody fragment or subfragment by detecting elevated levels of the labeled antibody fragment or subfragment at such sites with the detection means, without the use of a clearing agent, contrast agent or subtraction agent, and optionally, treating the detected sites of accretion of said labeled antibody fragment or subfragment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. A method of detection of lesions during an endoscopic, intravascular catheter or surgical procedure, wherein the method comprises the steps of:
-
injecting a patient to undergo such a procedure with an antibody fragment or subfragment labeled with a fluorescent agent or dye, wherein the labeled antibody fragment or subfragment accretes at the lesion; permitting the labeled antibody fragment or subfragment to accrete; conducting the procedure within 48 hours of the injection; and detecting the label with a light source supplied via an endoscope or intravascular catheter or during the surgical procedure, without the use of a clearing agent, contrast agent or subtraction agent. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
-
-
30. A method of detection and treatment of lesions during an endoscopic or intravascular catheter procedure, wherein the method comprises the steps of:
-
(a) injecting a patient to undergo such a procedure with an antibody fragment or subfragment labeled with an agent capable of detection, which labeled antibody fragment or subfragment preferentially accretes at the lesion; (b) permitting the labeled antibody fragment or subfragment to accrete at the lesion; (c) conducting the procedure within 48 hours of the injection of the labeled antibody fragment or subfragment; (d) detecting the agent with a detection means supplied via the endoscope or intravascular catheter, wherein the agent is detected without the use of a clearing agent, contrast agent or subtraction agent; and (e) treating the lesion by brachytherapy administered through the endoscope or intravascular catheter. - View Dependent Claims (31, 32, 33, 34)
-
-
35. A method of treatment of lesions during a laparoscopic or intravascular catheter procedure, wherein the method comprised the steps of:
-
injecting a patient to undergo such a procedure with an antibody fragment or subfragment labeled with a photoactive agent, wherein the antibody fragment or subfragment preferentially accretes at targeted lesions; permitting the labeled antibody fragment or subfragment to accrete; conducting the procedure within 48 hours of the injection of the labeled antibody or subfragment; and activating the photoactive agent with a light source supplied via the laparascope or intravascular catheter, thereby treating said lesions. - View Dependent Claims (36, 37)
-
-
38. A method of treatment of lesions, wherein the method comprises the steps of:
-
(a) injecting a patient with composition comprising a protein conjugated to an agent capable of being activated to emit Auger electrons or other ionizing radiation, and, optionally, to an agent capable of detection, wherein the protein conjugate accretes preferentially at the targeted lesion; and (b) activating said agent capable of being activated, thereby treating said lesions, and, optionally, detecting the optional agent capable of detection. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
-
Specification