Method and apparatus for desorption and ionization of analytes
First Claim
1. A method for desorbing analyte molecules from a sample presenting surface, comprising:
- providing a sample presenting surface derivitized with an affinity capture device having means for binding with said analyte molecules;
exposing said derivitized sample presenting surface to a sample containing said analyte molecules;
capturing said analyte molecules on said derivitized sample presenting surface by means of said affinity capture device; and
exposing said analyte molecules, while bound to said derivitized sample presenting surface by means of said affinity capture device, to an energy or light source to desorb, without substantial combustion at least a portion of said analyte molecules from said surface.
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Abstract
This invention relates generally to methods and apparatus for desorption and ionization of analytes for the purpose of subsequent scientific analysis by such methods, for example, as mass spectrometry or biosensors. More specifically, this invention relates to the field of mass spectrometry, especially to the type of matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry used to analyze macromolecules, such as proteins or biomolecules. Most specifically, this invention relates to the sample probe geometry, sample probe composition, and sample probe surface chemistries that enable the selective capture and desorption of analytes, including intact macromolecules, directly from the probe surface into the gas (vapor) phase without added chemical matrix.
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Citations
20 Claims
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1. A method for desorbing analyte molecules from a sample presenting surface, comprising:
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providing a sample presenting surface derivitized with an affinity capture device having means for binding with said analyte molecules; exposing said derivitized sample presenting surface to a sample containing said analyte molecules; capturing said analyte molecules on said derivitized sample presenting surface by means of said affinity capture device; and exposing said analyte molecules, while bound to said derivitized sample presenting surface by means of said affinity capture device, to an energy or light source to desorb, without substantial combustion at least a portion of said analyte molecules from said surface. - View Dependent Claims (2, 3, 4, 5, 7, 8, 9)
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6. The method according to claim or 1, wherein the binding is non-selective.
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10. A method for preparing a sample probe for mass spectrometry for presenting analyte molecules for analysis, said method comprising:
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providing a substrate on said surface for supporting said analyte; derivitizing said substrate with an affinity capture device having means for selectively binding with said analyte.
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11. A sample probe for promoting desorption of intact analytes into the gas phase comprising:
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a sample presenting surface; and an affinity capture device associated with said sample presenting surface;
wherein, when said sample probe is impinged by an energy source, said sample probe promotes the transition of an intact analyte molecule into the gas phase. - View Dependent Claims (12, 13, 14, 19, 20)
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15. A sample probe for promoting differential desorption of intact analyte into the gas phase, comprising:
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a sample presentation surface; and at least two different affinity capture devices associated with said sample presentation surface;
wherein, when said sample probe is impinged by an energy source, said sample probe promotes the transition of an analyte molecule into the gas phase at different rates depending on the affinity capture device associated with said analyte molecule. - View Dependent Claims (16, 17)
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18. A sample probe for presenting an analyte to an energy source capable of desorbing the analyte from the probe, comprising:
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a sample presentation surface; and a surface associated molecule associated with said sample presenting surface, wherein said surface associated molecule can function both as an energy absorbing molecule and as an affinity capture device.
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Specification