Reaction container arrangement for use in a thermal cycler

• US 5,720,406 A
• Filed: 08/16/1996
• Issued: 02/24/1998
• Est. Priority Date: 09/10/1993
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Corporation (Roche Holding AG)
• Original Assignee: Roche Diagnostic Systems Incorporated (Roche Holding AG)
• Inventors: Fassbind, Walter, Japichino, Emanuele
• Primary Examiner(s): MOY, JOSEPH MAN
• Application Number:
  US08/698,659
• Time in Patent Office: 557 Days
• Field of Search: 220/23.2, 220/23.4, 220/23.83, 220/23.86, 220/604, 220/669
• US Class Current: 220/23.4
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A throwaway arrangement of reaction containers each having the same shape and dimensions for bringing about temperature cycles in a liquid mixture contained within the reaction containers. Each reaction container having a first conical wall region, and a second cylindrical wall region which at one end forms the opening of the reaction container. The thickness of the first wall region is less than the thickness of the second wall region. The opening of the reaction container is adapted to receive a cover for closing the container in gas-tight manner when placed on the opening of the reaction container. To facilitate handling and access to the liquids in the reaction containers, the arrangement reaction containers is annular, and the closure of each reaction container can be pierced by a pipetting needle.

Claims

1. An arrangement of reaction containers for bringing about temperature cycles in a liquid mixture contained within the reaction containers, which comprises:

(a) a plurality of reaction containers each having substantially the same shape and dimensions, each reaction container having

(i) a first conical wall region, and

(ii) a second cylindrical wall region adjacent the first conical wall region, and open at one end to form the opening of the reaction container, the thickness of the first wall region being less than the thickness of the second wall region; and

(b) a plurality of closures, each closure being configured and dimensioned to fit within the opening of a reaction container and close the reaction container in a gas-tight manner, each closure being configured and dimensioned to be piercable by a pipetting needle.

2. The arrangement according to claim 1, wherein the plurality of reaction containers are made in one piece from a plastic, and adjacent reaction containers are joined by a flexible web.

3. The arrangement according to claim 2, wherein the plurality of closures are made in one piece from the same plastic as the plurality of reaction containers, adjacent closures in the arrangement being joined by at least one flexible web.

4. The arrangement according to claim 2, wherein the plurality of reaction containers are arranged as a continuous loop.

5. The arrangement according to claim 3, wherein the plurality of closures are arranged as a continuous loop.

6. The arrangement according to claim 1, wherein the plurality of reaction containers are made in one piece from a plastic and form at least a segment of a ring, with adjacent reaction containers being joined by a flexible web to form an assembly of reaction containers.

7. The arrangement of reaction containers according to claim 6, wherein the plurality of closures forms at least a segment of a ring and is made in one piece from the same plastic as the reaction containers, adjacent closures in the arrangement being joined by at least one flexible web to form an assembly of closures with each closure being adapted to close one reaction container in gas-tight manner when placed in the opening thereof, the arrangement being assembled by juxtaposing the assembly of reaction containers with the assembly of closures.

8. The arrangement according to claim 6, wherein the arrangement of the plurality of reaction containers forms a complete ring.

9. The arrangement according to claim 7, wherein the plurality of closures forms a complete ring.

11. The arrangement according to claim 3, wherein that the arrangement has an extension for positioning or detecting the arrangement.

12. The arrangement according to claim 7, wherein that the arrangement has an extension for positioning or detecting the arrangement.

10. An arrangement of reaction containers for bringing about temperature cycles in a liquid mixture contained within the reaction containers, which comprises:

(a) a plurality of reaction chambers each having substantially the same shape and dimensions, being made in one piece from a plastic with each reaction container being joined to at least one other reaction container by a flexible web, each reaction container having

(i) a first conical wall region, and

(ii) a second cylindrical wall region adjacent the first conical wall region and open at one end to form the opening of the reaction container, the thickness of the first wall region being less than the thickness of the second wall region; and

(b) a plurality of closures made in one piece from the same plastic as the reaction containers, each closure being joined to at least one other closure by at least one flexible web, each closure being piercable by a pipetting needle, and configured and dimensioned to fit within the opening of a reaction container and close the reaction container in gas-tight manner.

13. The arrangement according to claim 10, wherein that the arrangement has an extension for positioning or detecting the arrangement.

14. In a thermal cycler for bringing about temperature cycles in a liquid mixture contained within an arrangement of reaction containers, the improvement comprising (a) the thermal cycler having a pipetting needle and (b) the reaction containers each having (1) a first conical wall region, (2) a second cylindrical wall region adjacent the first conical wall region, the thickness of the first wall region being less than the thickness of the second wall region;

and (3) a closure configured and dimensioned to seal the reaction container in a gas-tight manner, each closure being configured and dimensioned to be piercable by the pipetting needle.