Process for the production of novel pullulanase
First Claim
1. A process for the production of isolated Bacillus deramificans pullulanase or a derivative or a mutant thereof, wherein said pullulanase or said derivative or said mutant catalyses hydrolysis of α
- -1,6-glucosidic bonds and comprises an N terminal sequence of Seq. ID No.;
1, comprising the steps of;
culturing the species Bacillus deramificans or a derivative or a mutant of Bacillus deramificans that retains all identifying characteristics of said species which produces said pullulanase in a suitable nutrient medium containing sources of carbon and nitrogen and mineral salts under aerobic conditions, and harvesting of the pullulanase obtained.
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Accused Products
Abstract
The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the α-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60° C.
The invention also relates to strains of micro-organisms which produce this pullulanase and processes for the preparation of this pullulanase.
The invention also relates to the uses thereof and compositions comprising the product.
The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.
40 Citations
1 Claim
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1. A process for the production of isolated Bacillus deramificans pullulanase or a derivative or a mutant thereof, wherein said pullulanase or said derivative or said mutant catalyses hydrolysis of α
- -1,6-glucosidic bonds and comprises an N terminal sequence of Seq. ID No.;
1, comprising the steps of;culturing the species Bacillus deramificans or a derivative or a mutant of Bacillus deramificans that retains all identifying characteristics of said species which produces said pullulanase in a suitable nutrient medium containing sources of carbon and nitrogen and mineral salts under aerobic conditions, and harvesting of the pullulanase obtained.
- -1,6-glucosidic bonds and comprises an N terminal sequence of Seq. ID No.;
Specification
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- Resources
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Current AssigneeNEC Electronics Corporation (Renesas Electronics Corporation)
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Original AssigneeGenencor International Incorporated (International Flavors & Fragrances Incorporated)
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InventorsAmory, Antoine, Deweer, Philippe
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Primary Examiner(s)Wax, Robert A.
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Assistant Examiner(s)HOBBS, LISA JOE
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Application NumberUS08/477,630Time in Patent Office993 DaysField of Search435/210US Class Current435/210CPC Class CodesC12N 1/205 Bacterial isolatesC12N 9/2451 acting on alpha-1,6-glucosi...C12N 9/2457 Pullulanase (3.2.1.41)C12P 19/16 produced by the action of a...C12R 2001/07 BacillusC12Y 302/01041 Pullulanase (3.2.1.41)
