Sealing device for thermal cycling vessels

• US 5,721,136 A
• Filed: 11/09/1994
• Issued: 02/24/1998
• Est. Priority Date: 11/09/1994
• Status: Expired due to Term

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: MJ Research Incorporated (Bio-Rad Laboratories Incorporated)
• Inventors: Titcomb, Paul, Finney, Michael J.
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US08/337,160
• Time in Patent Office: 1,203 Days
• Field of Search: 435/286.1, 435/287.2, 435/288.1, 435/288.2, 435/288.4, 435/303.1, 435/305.1-305.4, 435/288.3, 422/99, 422/101, 422/102, 220/23.2, 220/23.4, 220/23.8, 220/23.83, 220/523, 220/526, 220/255, 220/359, 220/378, 100/54, 100/92, 100/211, 428/40, 428/163, 428/167, 428/172, 428/411.1, 428/446, 428/447, 428/450, 428/451, 428/465, 359/396, 359/398, 156/69
• US Class Current: 435/287.2
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: Y10T 428/214 End closure
• CPC Class Codes: Y10T 428/28 Web or sheet containing structurally defined element or component and having an adhesive outermost layer
• CPC Class Codes: Y10T 428/2835 including moisture or waterproof component
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane

Abstract

A sheet of material seals vessels for biochemical reactions which undergo thermal cycling, and comprises a multilayer composite sheet which is placed over the openings of one or more reaction vessels. The multilayer composite sheet material has at least two layers, one layer providing strength and integrity to the material and the second layer being relatively thick comprising a deformable substance with a tacky surface to contact and seal vessels for biochemical reactions.

Claims

1. A multilayer composite material for contacting and sealing an open end of at least one reaction vessel wherein the reaction vessel has a closed end and an open end, comprising:

a first generally planar layer formed of a deformable material for contact with the open end of the at least one reaction vessel;

a second generally planar layer formed of a material which provides support to the first layer;

the first and the second layers being joined together to form a multilayer composite;

wherein the first-mentioned layer is constructed of a material with hardness less than 30 Shore A and possesses a tacky surface on each side of the planar layer having a peel strength or polyporpylene of at least 0.1 gf/cm but no more than 50 gf/cm.

2. The multilayer material of claim 1 wherein the first-mentioned material will maintain its integrity up to an applied force of approximately 100 gf/sq. cm.

3. The multilayer material of claim 1 wherein the first-mentioned material is comprised of a material which is inelastically deformable.

4. The multilayer material of claim 1 wherein the first-mentioned material is comprised of a material which is elastically deformable.

5. The multilayer material of claim 1 wherein the first-mentioned layer is between approximately 0.05 mm and 1.0 mm in thickness.

6. The multilayer material of claim 1 wherein the first-mentioned layer is comprised of an uncured silicone compound.

7. The multilayer material of claim 1 wherein the first-mentioned layer is comprised of a silicone gel.

8. The multilayer material of claim 1 wherein the first-mentioned layer contains internal gaps formed therein.

9. The multilayer material of claim 1 wherein the second-mentioned layer is comprised of a material which maintains its integrity at 1 atmosphere of pressure and 100 degrees temperature C.

10. The multilayer material of claim 1 wherein the second-mentioned layer is comprised of a material which is essentially impermeable to water.

11. The multilayer material of claim 1 wherein the second-mentioned layer is comprised of a material which is flexible.

12. The multilayer material of claim 1 wherein the second-mentioned layer is formed from a polyester film.

13. The multilayer material of claim 1 wherein the second-mentioned layer is between approximately 0.03 mm to 0.1 mm in thickness.

14. In an apparatus for thermally cycling reaction materials contained in at least one reaction vessel, the at least one reaction vessel having a closed end and an open end, the apparatus further including a temperature control block for supporting the at least one reaction vessel at its closed end, a flat heated plate disposed above the open end of the at least one reaction vessel, wherein the improvement comprises a multilayer composite material for sealing the open end of reaction vessels having:

a first generally planar layer formed of a deformable material for contact with the open end of a reaction vessel, a second generally planar layer formed of a material which provides support to the first layer, the first and the second layers being joined together to form a unitary multilayer composite, wherein in the first-mentioned layer is constructed of a material with hardness less than 30 Shore A and possess a tacky surface having a peel strength or polypropylene of at least 0.1 gf/cm on each side of the planar layer but no more than 50 gf/cm;

the multilayer composite being disposed between the flat heated plate and the open end of the at least one reaction vessel;

the first mentioned layer being deformed and sealing the open end of the at least one reaction vessel when the deformable surface of the composite is compressed onto the open end by the flat heated plate.