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Homologous recombination in mammalian cells

  • US 5,721,367 A
  • Filed: 06/05/1995
  • Issued: 02/24/1998
  • Est. Priority Date: 08/29/1990
  • Status: Expired due to Term
First Claim
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1. A method for generating a DNA segment inside a cell comprising the step of introducing at least a first and a second DNA fragment into a recombination competent mammalian cell, each of said DNA fragments having a 5'"'"' and a 3'"'"' sequence portion, said first DNA fragment 3'"'"' sequence portion having at least 80% sequence identity to said second DNA fragment 5'"'"' sequence portion, wherein said first and second DNA fragment homologously recombine in said cell to form are combined DNA segment greater than 50 kb in length, said recombined DNA segment being integrated into the genome of said cell.

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