Process for detecting an antibody using a nucleic acid amplification probe

• US 5,723,297 A
• Filed: 05/28/1996
• Issued: 03/03/1998
• Est. Priority Date: 09/10/1991
• Status: Expired due to Fees

• Current Assignee: GEN Trak, Inc.
• Original Assignee: GEN Trak, Inc.
• Inventors: Love, Jack D.
• Primary Examiner(s): Campbell, Eggerton A.
• Application Number:
  US08/654,243
• Time in Patent Office: 644 Days
• Field of Search: 435/6, 435/91.2, 435/7.1, 935/77, 935/78
• US Class Current: 435/6.18
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6804 Nucleic acid analysis using immunogens immunoassay G01N33/53
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6865 Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
• CPC Class Codes: C12Q 2521/119 RNA polymerase
• CPC Class Codes: C12Q 2525/121 incorporating both deoxyribonucleotides and ribonucleotides
• CPC Class Codes: C12Q 2525/143 incorporating a promoter sequence
• CPC Class Codes: C12Q 2531/143 Promoter based amplification, e.g. NASBA, 3SR, TAS
• CPC Class Codes: C12Q 2533/101 Primer extension
• CPC Class Codes: C12Q 2563/131 the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
• CPC Class Codes: C12Q 2563/179 the label being a nucleic acid
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target
• CPC Class Codes: G01N 33/6854 Immunoglobulins

Abstract

A process for detecting the presence or absence of a specific nucleic acid sequence or antibody in a sample using an oligonucleotide to bind to the nucleic acid sequence or antibody to be detected, forming double-stranded nucleic acid sequence using the bound oligonucleotide in conjunction with another oligonucleotide or DNA synthesis, synthesizing RNA transcripts from the thus-formed double-stranded nucleic acid sequence, and detecting the existence of the RNA transcripts, and oligonucleotides and kits useful in carrying out such a process.

Claims

1. A process for detecting the presence or absence of at least one specific antibody in a sample containing an antibody or mixture of antibodies, which process comprises:

(a) contacting the sample with an antigen which binds to said antibody to form an antibody-antigen complex,

(b) treating the sample to remove any of said antibody or said antigen which are not bound,

(c) treating the sample with a first oligonucleotide under conditions such that a portion of said first oligonucleotide binds to said antibody-antigen complex and a portion of said first oligonucleotide does not bind to said antibody-antigen complex, wherein said portion of said first oligonucleotide which does not bind to said antibody-antigen complex forms a double-stranded RNA polymerase binding site and a nucleotide sequence transcribing RNA when hybridized to a complementary nucleotide sequence in the absence of nucleic acid extension,

(d) treating the sample to remove any of first oligonucleotide which is not bound to said antibody-antigen complex,

(e) treating the sample with a second oligonucleotide under hybridizing conditions such that said second oligonucleotide hybridizes to said portion of said first oligonucleotide which does not bind to said antibody-antigen complex and forms a double-stranded RNA polymerase binding site and nucleotide sequence transcribing RNA,

(f) treating the sample with RNA polymerase and nucleotide triphosphates such that said nucleotide sequence formed between a portion of said first oligonucleotide and a portion of said second oligonucleotide results in the synthesis of RNA transcripts from the nucleotide sequence transcribing RNA, and

(g) determining whether said RNA transcripts were synthesized.

2. The process of claim 1, wherein the sample at step (g) is further treated with said first oligonucleotide and with DNA polymerase such that said first oligonucleotide hybridizes to said RNA transcripts which act as primers for synthesis of an extension nucleotide sequence complementary to said first oligonucleotide to form a double-stranded RNA polymerase binding site and a nucleotide sequence transcribing RNA which results in the synthesis of additional RNA transcripts.

3. The process of claim 2, wherein the step of treating the sample with said first oligonucleotide and with DNA polymerase is repeated.

4. The process of claim 1, wherein said RNA transcripts are virus fragments and are replicated through use of an RNA virus replicase.

5. The process of claim 1, wherein said first oligonucleotide is biotinylated and binds to said antibody-antigen complex through a streptavidin-complexed molecule.

6. The process of claim 1, wherein said second oligonucleotide acts as a primer for said first oligonucleotide and the RNA polymerase binding site and nucleotide sequence transcribing RNA are formed by the addition of DNA polymerase to the sample such that an extension product is synthesized from said primer which is complementary to said first oligonucleotide sequence.