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Method of identifying a stochastically-generated peptide, polypeptide, or protein having ligand binding property and compositions thereof

DC
  • US 5,723,323 A
  • Filed: 12/02/1994
  • Issued: 03/03/1998
  • Est. Priority Date: 03/30/1985
  • Status: Expired due to Term
First Claim
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1. A method of identifying a peptide, polypeptide or protein having a binding property to a ligand, comprising:

  • (a) providing a ligand for detecting said binding property;

    (b) synthesizing a diverse population of stochastically generated polynucleotide sequences;

    (c) inserting said diverse population of stochastically generated polynucleotide sequences into a population of expression vectors to form a diverse population of expression vectors containing stochastically generated polynucleotide sequences;

    (d) expressing in host cells said diverse population of expression vectors containing stochastically generated polynucleotide sequences to produce a diverse population of peptides, polypeptides or proteins; and

    (e) screening said diverse population of peptides, polypeptides or proteins with said ligand under conditions which allow binding and detection of one or more peptides, polypeptides or proteins having said predetermined property.

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