Method of identifying a stochastically-generated peptide, polypeptide, or protein having ligand binding property and compositions thereof
DCFirst Claim
1. A method of identifying a peptide, polypeptide or protein having a binding property to a ligand, comprising:
- (a) providing a ligand for detecting said binding property;
(b) synthesizing a diverse population of stochastically generated polynucleotide sequences;
(c) inserting said diverse population of stochastically generated polynucleotide sequences into a population of expression vectors to form a diverse population of expression vectors containing stochastically generated polynucleotide sequences;
(d) expressing in host cells said diverse population of expression vectors containing stochastically generated polynucleotide sequences to produce a diverse population of peptides, polypeptides or proteins; and
(e) screening said diverse population of peptides, polypeptides or proteins with said ligand under conditions which allow binding and detection of one or more peptides, polypeptides or proteins having said predetermined property.
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Abstract
The present invention is directed to a process for the production of a peptide, polypeptide, or protein having a predetermined property. In accordance with one embodiment, the process begins by producing by way of synthetic polynucleotide coupling, stochastically generated polynucleotide sequences. A library of expression vectors containing such stochastically generated polynucleotide sequences is formed. Next, host cells containing the vectors are cultured so as to produce peptides, polypeptides, or proteins encoded by the stochastically generated polynucleotide sequences. Screening or selection is carried out on such host cells to identify a peptide, polypeptide, or protein produced by the host cells which has the predetermined property. The stochastically generated polynucleotide sequence which encodes the identified peptide, polypeptide, or protein is then isolated and used to produce the peptide, polypeptide, or protein having the predetermined property.
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Citations
48 Claims
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1. A method of identifying a peptide, polypeptide or protein having a binding property to a ligand, comprising:
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(a) providing a ligand for detecting said binding property; (b) synthesizing a diverse population of stochastically generated polynucleotide sequences; (c) inserting said diverse population of stochastically generated polynucleotide sequences into a population of expression vectors to form a diverse population of expression vectors containing stochastically generated polynucleotide sequences; (d) expressing in host cells said diverse population of expression vectors containing stochastically generated polynucleotide sequences to produce a diverse population of peptides, polypeptides or proteins; and (e) screening said diverse population of peptides, polypeptides or proteins with said ligand under conditions which allow binding and detection of one or more peptides, polypeptides or proteins having said predetermined property. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An isolated, diverse population of peptides, polypeptides, or proteins comprising greater than about 1×
- 105 different stochastic amino acid sequences encoded by stochastic polynucleotide sequences.
- View Dependent Claims (46, 47, 48)
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16. A method of isolating a polynucleotide sequence encoding a peptide, polypeptide or protein having a predetermined binding property to a ligand, comprising:
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(a) providing a ligand for detecting said binding property; (b) synthesizing a diverse population of stochastically generated polynucleotide sequences; (c) inserting said diverse population of stochastically generated polynucleotide sequences into a population of expression vectors to form a diverse population of expression vectors containing stochastically generated polynucleotide sequences; (d) expressing in host cells said diverse population of expression vectors containing stochastically generated polynucleotide sequences to produce a diverse population of peptides, polypeptides or proteins; (e) screening said diverse population of peptides, polypeptides or proteins with said ligand under conditions which allow binding and detection of one or more peptides, polypeptides or proteins to said ligand; and (f) isolating the stochastically generated polynucleotide sequence or sequences which encoding said peptides, polypeptides or proteins having said predetermined binding property to said ligand. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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24. An isolated, diverse population of polynucleotide sequences which encode a diverse population of ligand binding peptides, polypeptides, or proteins comprising greater than about 1×
- 105 different stochastic amino acid sequences encoded by the stochastic polynucleotide sequences.
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25. A method of isolating a peptide, polypeptide or protein having a binding property, comprising:
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(a), providing a ligand for detecting said binding property; (b) synthesizing a diverse population of stochastically generated polynucleotide sequences; (c) inserting said diverse population of stochastically generated polynucleotide sequences into a population of expression vectors to form a diverse population of expression vectors containing stochastically generated polynucleotide sequences; (d) expressing in host cells said diverse population of expression vectors containing stochastically generated polynucleotide sequences to produce a diverse population of peptides, polypeptides or proteins; (e) screening said diverse population of peptides, polypeptides or proteins with said ligand under conditions which allow binding and detection of one or more peptides, polypeptides or proteins having said predetermined property; (f) isolating the stochastically generated polynucleotide sequence or sequences encoding said peptides, polypeptides or proteins having said binding property to said ligand; and (g) using genetic information from said isolated stochastically generated polynucleotide sequence to produce said peptide, polypeptide or protein having said binding property. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33)
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34. A method of producing a diverse population of stochastically generated polynucleotide sequences encoding a diverse population of ligand binding peptides, polypeptides or proteins, comprising:
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(a) synthesizing a diverse population of stochastically generated polynucleotide sequences selected from a method consisting of stochastic copolymerization of double stranded oligonucleotides, copolymerization of the four kinds of nucleotide triphoshpates consisting of Adenine, Cytosine, Guanine and Thymine, and chemical synthesis; and (b) inserting said diverse population of stochastically generated polynucleotide sequences into a population of vectors to form a diverse population of vectors containing stochastically generated polynucleotide sequences. - View Dependent Claims (35, 36, 37, 38, 39, 40)
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41. An isolated, diverse population of vectors comprising stochastically generated polynucleotide sequences encoding a diverse population of ligand binding peptides, polypeptides, or proteins comprising greater than about 1×
- 105 different stochastic amino acid sequences.
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42. An isolated, diverse population of peptides or polypeptides comprising stochastic amino acid sequences encoded by stochastic polynucleotide sequences of about 300 nucleotides or less in length.
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43. An isolated, diverse population of polynucleotides sequences encoding a diverse population of ligand binding peptides or polypeptides comprising stochastic amino acid sequences encoded by stochastic polynucleotide sequences of about 300 nucleotides or less in length.
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44. An isolated, diverse population of vectors encoding a diverse population of ligand binding peptides or polypeptides comprising stochastic amino acid sequences encoded by stochastically generated polynucleotide sequences of about 300 nucleotides or less in length.
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45. An isolated, diverse population of peptides, polypeptides, or proteins comprising stochastic amino acid sequences produced by a method comprising synthesizing a diverse population of stochastically generated polynucleotide sequences selected from a method consisting of stochastic copolymerization of double stranded oligonucleotides, copolymerization of the four kinds of nucleotide triphosphates consisting of Adenine, Cytosine, Guanine and Thymine, and chemical synthesis;
- and expressing said stochastically generated polynucleotide sequences.
Specification