Device for the capture of target molecules, and capturing process using the device
First Claim
1. Device for capturing a target molecule for the purpose of detecting it and/or assaying it, comprising a water-soluble conjugate immobilized on a solid support by adsorption, said conjugate resulting from a covalent coupling of a polymer with a plurality of molecules of a ligand, wherein said polymer is an N-vinylpyrrolidone copolymer.
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Accused Products
Abstract
Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption.
When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.
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Citations
15 Claims
- 1. Device for capturing a target molecule for the purpose of detecting it and/or assaying it, comprising a water-soluble conjugate immobilized on a solid support by adsorption, said conjugate resulting from a covalent coupling of a polymer with a plurality of molecules of a ligand, wherein said polymer is an N-vinylpyrrolidone copolymer.
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13. Kit for capturing a target molecule for the purpose of detecting and/or assaying it, comprising:
- a container containing (1) a water-soluble conjugate immobilized on a solid support by adsorption, said conjugate resulting from a covalent coupling of a polymer with a plurality of molecules of a ligand, wherein said polymer is an N-vinyl pyrrolidone copolymer, and (2) a bifunctional reagent comprising an anti-ligand group bonded with a partner group, wherein said anti-ligand group binds with the ligand to form a complex and said partner group binds with the target to form a partner/target complex.
- View Dependent Claims (14)
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15. A process for capturing a target molecule, comprising:
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providing a device comprising;
(1) a water-soluble conjugate immobilized on a solid support by adsorption, said conjugate resulting from a covalent coupling of an N-vinylpyrrolidone copolymer with a plurality of molecules of a ligand, and (2) a bifunctional reagent comprising an anti-ligand group bonded with a partner group, wherein said anti-ligand group is attached to a ligand of the water-soluble conjugate to form a complex;bringing the device into contact with the target molecule in solution in an aqueous medium under conditions permitting the formation of a complex between the partner group and the target; and allowing said complex between the partner and the target to form.
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Specification