High affinity PDGF nucleic acid ligands

• US 5,723,594 A
• Filed: 03/20/1996
• Issued: 03/03/1998
• Est. Priority Date: 06/07/1995
• Status: Expired due to Term

• Current Assignee: Gilead Sciences Inc.
• Original Assignee: NeXstar Pharmaceuticals, Inc. (Gilead Sciences Inc.)
• Inventors: Janjic, Nebojsa, Gold, Larry
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US08/618,693
• Time in Patent Office: 713 Days
• Field of Search: 536/23.1, 536/25.4, 435/6, 435/91.2, 935/77, 935/78
• US Class Current: 536/23.1
• CPC Class Codes: C07K 14/49 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C07K 14/495 Transforming growth factor [TGF]
• CPC Class Codes: C07K 14/50 Fibroblast growth factor [FGF]
• CPC Class Codes: C12N 15/115 Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
• CPC Class Codes: C12Q 1/6811 Selection methods for production or design of target specific oligonucleotides or binding molecules
• CPC Class Codes: G01N 2333/96433 Serine endopeptidases (3.4.21)

Abstract

Methods are described for the identification and preparation of high-affinity nucleic acid ligands to PDGF. Included in the invention are specific ssDNA and RNA ligands to PDGF identified by the SELEX method.

Claims

1. A purified and isolated non-naturally occurring DNA ligand to Platelet Derived Growth Factor (PDGF) wherein said ligand is selected from the group consisting of SEQ ID NOS:

82-87.

3. The purified and isolated non-naturally occurring DNA ligand to PDGF of claim 1 consisting of the nucleotide sequence of SEQ ID NO:

87.

2. A purified and isolated non-naturally occurring nucleic acid ligand to PDGF comprising the conserved structure shown in FIG. 1.