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Reversible flow chromatographic binding assay

  • US 5,726,010 A
  • Filed: 07/31/1991
  • Issued: 03/10/1998
  • Est. Priority Date: 07/31/1991
  • Status: Expired due to Term
First Claim
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1. A device for performing an assay which determines the presence or quantity of an analyte in a fluid sample by detecting binding of said analyte to at least one immobilized analyte capture reagent after washing unbound material from said immobilized analyte capture reagent, said device comprising:

  • (a) an elongated solid phase flow matrix, said flow matrix comprising capillary channels capable of driving capillary fluid movement, said flow matrix further comprisingi) a first region adapted for receipt of said fluid sample,ii) a second region at which said analyte capture reagent is immobilized,iii) a third region for application of a liquid detector reagent capable of removing unbound substances from said second region;

    said second region being positioned intermediate to said first region and said third region;

    (b) an absorbent reservoir of high volume capacity, wherein, prior to use of said device, said absorbent reservoir is not in fluidic contact with said flow matrix;

    said device further comprising means for establishing fluidic contact between said absorbent reservoir and said flow matrix at a position selected so that said second region is between said absorbent reservoir and said third region;

    (c) a sealed container of said liquid detector reagent positioned to be introduced at said third region of said matrix;

    whereby said flow matrix and said regions thereof are sized and positioned to cause said fluid sample to flow initially along said elongated flow matrix in one direction toward and through said second region, and subsequently, upon introduction of said liquid detector reagent into the third region of said flow matrix, said liquid detector reagent to flow along said elongated flow matrix in a second direction opposite said first direction, through said second region, and into said absorbent reservoir, drawing unbound substances with it.

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