High affinity HIV-1 gag nucleic acid ligands
First Claim
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1. A method for identifying nucleic acid ligands to HIV-1 GAG, comprising:
- a) preparing a candidate mixture of nucleic acids;
b) contacting the candidate mixture of nucleic acids with HIV-1 GAG, wherein nucleic acids having an increased affinity to HIV-1 GAG relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to HIV-1 GAG, whereby nucleic acids ligands to HIV-1 GAG may be identified.
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Abstract
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to HIV-1 GAG, Included in the invention are specific RNA ligands to HIV-1 GAG identified by the SELEX method, Also included are RNA ligands that inhibit the function of HIV-1 GAG.
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10 Claims
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1. A method for identifying nucleic acid ligands to HIV-1 GAG, comprising:
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a) preparing a candidate mixture of nucleic acids; b) contacting the candidate mixture of nucleic acids with HIV-1 GAG, wherein nucleic acids having an increased affinity to HIV-1 GAG relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to HIV-1 GAG, whereby nucleic acids ligands to HIV-1 GAG may be identified. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A purified and isolated non-naturally occurring RNA ligand to HIV-1 GAG wherein said ligand is selected from the group consisting of the sequences set forth in SEQ ID NOS. 8-27 and 34-46.
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10. A purified and isolated non-naturally occurring nucleic acid ligand to HIV-1 GAG.
Specification