Sample introduction apparatus for a flow cytometer

• US 5,726,364 A
• Filed: 02/10/1997
• Issued: 03/10/1998
• Est. Priority Date: 10/14/1994
• Status: Expired due to Term

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: University of Washington
• Inventors: Van den Engh, Ger
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US08/798,177
• Time in Patent Office: 393 Days
• Field of Search: 73/864.85, 73/864.86, 73/864.84, 73/864.87
• US Class Current: 73/864.85
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

A sample introduction system for a flow cytometer allows easy change of sample containers such as test tubes and facilitates use in high pressure environments. The sample container includes a cap having a pressure supply chamber and a sample container attachment cavity. A sample container may be automatically positioned into the attachment cavity so as to sealably engage the end of the sample container as its outer surface. This positioning may be accomplished through some sample introduction mechanism. To facilitate cleaning HPLC tubing and fittings may be used in a manner which facilitates removable of the entire tubing from both the nozzle container and other sample container cap to permit its replacement to avoid contamination. The sample container support may include horizontal stops which loosely limit the movement of the sample container and thus avoid further stresses upon it.

Claims

1. A sample introduction system for a flow cytometer comprising:

a. a nozzle container establishing a nozzle volume;

b. a sheath fluid port located within said nozzle volume;

c. a substance introduction port located within said nozzle volume;

d. a substance supply tube to which said substance introduction port is responsive;

e. a sample container cap attached to said substance supply tube and having an inner surface;

f. a sample container which serves as a reservoir for a substance having an outer surface;

g. an external seal responsive to the inner surface of said sample container cap and responsive to the outer surface of said sample container;

h. a pressure source to which said sample container cap is responsive.

2. A sample introduction system for a flow cytometer as described in claim 1 and further comprising:

a. a sample container support positioned below said sample container cap; and

b. a sample introduction mechanism which varies the relationship between said sample container support and said sample container cap.

3. A sample introduction system for a flow cytometer as described in claim 2 wherein said sample container has a bottom and wherein said sample container support comprises:

a. a vertical support on which said sample container bottom is positioned; and

b. a horizontal stop attached to said vertical support so as to be generally detached from said sample container bottom and which allows said sample container bottom to move in a horizontal plane.

4. A sample introduction system for a flow cytometer as described in claim 3 wherein said sample introduction mechanism comprises an arm attached to said sample container support.

5. A sample introduction system for a flow cytometer as described in claim 1 wherein said substance supply tube is sealed to and removable from both said sample container cap and said nozzle container.

6. A sample introduction system for a flow cytometer as described in claim 3 wherein said substance supply tube is sealed to and removable from both said sample container cap and said nozzle container.

7. A sample introduction system for a flow cytometer as described in claim 1 wherein said sample container cap has an axis and wherein said pressure source comprises an angled pressure source to which said sample container cap is responsive and which supplies a pressure at an angle to said axis.

8. A sample introduction system for a flow cytometer as described in claim 7 wherein said angle is about 90 degrees.

9. A sample introduction system for a flow cytometer as described in claim 7 wherein said axis is vertical.

11. A sample introduction system for a flow cytometer as described in claim 9 wherein said sample container cap has an axis and wherein said pressure source comprises an angled pressure source which supplies a pressure at an angle to said axis.

12. A sample introduction system for a flow cytometer as described in claim 11 wherein said angle is about 90 degrees.

13. A sample introduction system for a flow cytometer as described in claim 11 wherein said sample container has a bottom and wherein said sample container support comprises:

a. a vertical support on which said sample container bottom is positioned; and

b. a horizontal stop attached to said vertical support so as to be generally detached from said sample container bottom and which allows said sample container bottom to move in a horizontal plane.

14. A sample introduction system for a flow cytometer as described in claim 11 wherein said axis is vertical.

10. A sample introduction system for a flow cytometer comprising:

a. a nozzle container establishing a nozzle volume;

b. a sheath fluid port located within said nozzle volume;

c. a substance introduction port located within said nozzle volume;

d. a flexible substance supply tube having first and second ends, removably connected at said first end to said nozzle container, and to which said substance introduction port is responsive;

e. a sample container cap removably connected to said flexible substance supply tube, wherein said sample container cap comprises;

i) a sample container cap inner surface forming a sample container attachment cavity and a pressure supply chamber; and

ii) an O-ring seal attached to said sample container cap inner surface;

and wherein said substance supply tube passes through said sample container cap;

f. a sample container support situated below said sample container cap and having one or more attachment points thereon;

g. a sample container which serves as a reservoir for a substance having an outer surface and a top end, wherein said sample container is responsive to said sample container support, and into which said second end of said substance supply tube is positioned;

h. a sample introduction mechanism which is attached to said one or more attachment points on said sample container support, which positions said top end of said sample container into said sample container cap attachment cavity, and which sealably engages said O-ring seal against said outer surface of said sample container; and

i. a pressure source attached to said sample container cap at said pressure supply chamber of said sample container cap.

15. A sample introduction system for a flow cytometer as described in claim 10 wherein said sample container has a bottom and wherein said sample container support comprises:

a. a vertical support on which said sample container bottom is positioned; and

b. a horizontal stop attached to said vertical support so as to be generally detached from said sample container bottom and which allows said sample container bottom to move in a horizontal plane.

16. A sample introduction system for a flow cytometer comprising:

a. a nozzle container establishing a nozzle volume;

b. a sheath fluid port located within said nozzle volume;

c. a substance introduction port located within said nozzle volume;

d. a substance supply tube to which said substance introduction port is responsive;

e. a sample container cap having an axis, a pressure supply chamber, and attached to said substance supply tube;

f. a sample container responsive to said sample container cap; and

g. an angled pressure source to which said sample container cap is responsive and which supplies a pressure at an angle to said axis and within said pressure supply chamber.

17. A sample introduction system for a flow cytometer as described in claim 16 wherein said angle is about 90 degrees.

18. A sample introduction system for a flow cytometer as described in claim 16 wherein said axis is vertical.