Concentrator for separating small samples in a centrifuge

• US 5,728,267 A
• Filed: 11/15/1994
• Issued: 03/17/1998
• Est. Priority Date: 11/15/1994
• Status: Expired due to Fees

• Current Assignee: Flaherty, James E.
• Original Assignee: Flaherty, James E.
• Inventors: Flaherty, James E.
• Primary Examiner(s): FORTUNA, ANA M
• Application Number:
  US08/340,059
• Time in Patent Office: 1,218 Days
• Field of Search: 210/321.84, 210/380.1, 210/369, 210/407, 210/329, 210/781, 210/360.1, 210/978, 210/473, 210/321.67, 210/321.75, 454/36, 422/101
• US Class Current: 210/321.67
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01D 63/16 Rotary, reciprocated or vibrated modules
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: G01N 2001/4016 being a selective membrane, e.g. dialysis or osmosis

Abstract

A concentrator for concentrating a sample solution including molecules in solution which are larger than their molecular weight cutoff of a membrane in the concentrator. The concentrator has a sample reservoir which includes a bottom floor having an annular central wall surrounding a central opening. A membrane is held on a membrane support member positioned below the sample reservoir and a filtrate vial is held below the membrane to collect liquid which is passed through the membrane. A retentate vial is held over the top of the reservoir and after the sample has been concentrated the filtrate vial is removed and the remaining part of the assembly inverted. The assembly is then replaced in the centrifuge and spun to transfer the concentrated sample into the retentate vial. The membrane support member is made from spokes with open spaces between the spokes.

Claims

1. A concentrator for concentrating a sample solution including molecules in solution which are larger than the molecular weight cutoff of a membrane in the concentrator, said concentrator comprising:

a sample reservoir having a cylindrical side wall, an open top and a bottom including a bottom floor having a lower surface and an upper surface terminating in a central wall surrounding a central opening, said central wall having an annular crest parallel to said bottom floor and the volume below said annular crest and above said bottom floor being a concentrated sample well;

a membrane support member held below said bottom of said sample reservoir, said membrane support including a membrane support web positioned below said central opening in the bottom of said sample reservoir;

a membrane held on said membrane support web, said membrane held in a leak proof manner by membrane sealing means so that any liquid on the top of said membrane cannot pass around said membrane;

a generally cylindrical filtrate vial having an open top, a closed bottom, said filtrate vial being held by said membrane support member and positioned below said membrane so that filtrate passing through said membrane will be collected in said filtrate vial; and

a retentate vial held over the open top of said sample reservoir whereby a sample to be concentrated may be placed in said sample reservoir and the filtrate vial may be removed and the rest of the assembly may be placed in a centrifuge and spun so that the molecules in the sample which are smaller than the cutoff of the membrane will pass through the membrane but a small volume of concentrated sample will be held in the concentrated sample well after which the assembly may be inverted and spun again forcing the concentrated sample into the retentate vial.

2. The concentrator of claim 1 wherein the membrane support web comprises a plurality of spokes and said spokes form three equally spaced diametric bars with an open space between said bars.

3. The concentrator of claim 1 wherein said retentate vial has a generally conical floor surrounded by a cylindrical wall with a bottom edge which extends past said generally conical floor.

4. The concentrator of claim 3 wherein said cylindrical wall of the retentate vial has an outer surface and the outer surface of said cylindrical is serrated;

wherein the outer surface of the cylindrical wall of the retentate vial is threaded and the assembly further includes a cap with threads which may be screwed onto the retentate vial.

5. A concentrator for concentrating a sample solution including molecules in solution which are larger than the molecular weight cutoff of a membrane in the concentrator, said concentrator comprising:

a sample reservoir having a cylindrical side wall with a central axis, an open top and a bottom including a bottom floor having a lower surface and an upper surface terminating in a circular central wall concentrically located with respect to the central axis of said cylindrical side wall of said sample reservoir, said central wall surrounding a central opening, said central wall having an annular crest parallel to said bottom floor and the volume below said annular crest and above said bottom floor being a concentrated sample well;

a membrane support member held below said bottom of said sample reservoir, said membrane support including a membrane support web comprising a plurality of spokes with open spaces between the spokes, said membrane support positioned below said central opening in the bottom of said sample reservoir;

a circular membrane held on said membrane support web, said membrane held in a leak proof manner by membrane sealing means so that any liquid on the top of said membrane cannot pass around said membrane;

a generally cylindrical filtrate vial having an open top, a closed, generally conical bottom, said filtrate vial being ventingly held by said membrane support member and positioned below said membrane so that filtrate passing through said membrane will be collected in said filtrate vial; and

a retentate vial ventingly held over the open top of said sample reservoir whereby a sample to be concentrated may be placed in said sample reservoir and covered by said retentate vial and placed in a centrifuge and spun so that the molecules in the sample which are smaller than the cutoff of the membrane will pass through the membrane but a small volume of concentrated sample will be held in the concentrated sample well after which the fitrate vial is removed and the remaining assembly may be inverted and spun again forcing the concentrated sample into the retentate vial.

6. The concentrator of claim 5 wherein the membrane support comprises three diametric arms which are equally spaced from one another.

7. The concentrator of claim 6 wherein the filtrate vial has exterior threads near the top thereof and further including a filtrate cap with internal threads which match with the threads on the filtrate vial.