Apparatus for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures

• US 5,728,351 A
• Filed: 01/09/1995
• Issued: 03/17/1998
• Est. Priority Date: 01/21/1993
• Status: Expired due to Term

• Current Assignee: Drew Scientific Holdings, Inc.
• Original Assignee: CDC Technologies, Inc. (Escalon Medical Corporation)
• Inventors: Carver, Edward Lawrence Jr.
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US08/370,023
• Time in Patent Office: 1,163 Days
• Field of Search: 422/67, 422/73, 422/81, 422/100, 417/568
• US Class Current: 422/73
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

An apparatus is provided for hematology testing, which has a sensing unit defining a counting orifice for the flow of a blood sample through the counting orifice to analyze the blood sample, and a pump unit having three syringes. A first syringe is coupled in fluid communication with the sensing unit on the inlet side of the counting orifice for injecting a stream of blood sample through the counting orifice. A second syringe is coupled in fluid communication with the sensing chamber on the inlet side of the counting orifice for simultaneously injecting a sheath of fluid surrounding the sample stream on the inlet side of the counting orifice. And a third syringe is coupled to the sensing chamber on the outlet side of the counting orifice for aspirating a sheath of fluid from the sensing chamber surrounding the sample stream on the outlet side of the counting orifice. A control unit is connected to the pump unit for adjusting the reagent mixture to correspond to each of a plurality of different operator inputs, wherein each operator input may correspond to a respective animal species, to automatically create and analyze the reagent mixture for each animal species.

Claims

1. An apparatus for making a plurality of reagent mixtures for hematology testing and analyzing particle distributions within the reagent mixtures for blood cell analysis, comprising:

at least one pump;

a plurality of reagent chambers containing a plurality of reagent-mixture components, including at least a plurality of lysing agents, selectively connectable in fluid communication with the at least one pump;

a mixing chamber coupled in fluid communication with the at least one pump for receiving a plurality of reagent-mixture components and creating a reagent mixture therefrom;

a sensing unit defining a counting orifice for receiving the reagent mixture and analyzing a particle distribution of the reagent mixture; and

a control unit for adjusting the reagent mixture to correspond to each of a plurality of different operator inputs, the control unit being coupled to the at least one pump for controlling operation of the pump, and responsive to each respective operator input to;

(i) select one or more lysing agents from a database containing a plurality of lysing agents,

(ii) select a predetermined volume of the at least one selected lysing agent to correspond to said input,

(iii) pump the predetermined volume of the at least one selected lysing agent, and a predetermined volume of at least one other reagent-mixture component corresponding to said input into the mixing chamber to thereby create a reagent mixture corresponding to said input, and

(iv) introduce the reagent mixture from the mixing chamber through the counting orifice to analyze a particle distribution of the reagent mixture for blood cell analysis.

2. An apparatus as defined in claim 1, wherein the control unit is responsive to a respective operator input to control the at least one pump to introduce a predetermined volume of another reagent-mixture component corresponding to the respective input into the mixing chamber to thereby create a reagent mixture including predetermined volumes of first, second and third reagent-mixture components.

3. An apparatus as defined in claim 2, wherein a plurality of the operator inputs each specify a respective animal species, and for each such input the first, second and third reagent-mixture components are selected from the group including (i) a whole blood sample of the respective species, (ii) diluent, and (iii) a lysing agent, to make a blood/diluent/lyse reagent mixture corresponding to the respective species.

4. An apparatus as defined in claim 3, wherein the control unit is responsive to a respective operator input to control the at least one pump to introduce a predetermined volume of a second lysing agent corresponding to the respective species into the mixing chamber to make a blood/diluent/lyse reagent mixture corresponding to the respective species.

5. An apparatus as defined in claim 1, wherein at least a plurality of the operator inputs each correspond to a respective animal species, and the control unit includes a database pertaining to predetermined quantities of the lysing agents necessary for formulating reagent mixtures for each of the plurality of species, and wherein the control unit is responsive to each such input to control the at least one pump to:

(i) introduce the predetermined quantities of the reagent-mixture components corresponding to the respective species into the mixing chamber to thereby create a reagent mixture corresponding to the respective species, and

(ii) to control introduction of the reagent mixture through the counting orifice for analyzing a particle distribution of the reagent mixture for blood cell analysis.

6. An apparatus as defined in claim 1, wherein at least a plurality of the operator inputs each correspond to a respective species, and the apparatus further comprises a sample probe coupled to the at least one pump, the control unit being responsive to each such input to aspirate a predetermined volume of whole blood sample corresponding to the respective species into the probe.

7. An apparatus as defined in claim 6, wherein at least one reagent chamber contains diluent, and the control unit is responsive to each input selecting a particular species to aspirate a predetermined volume of diluent corresponding to the respective species to make a blood/diluent reagent mixture corresponding to the respective species.

8. An apparatus for making a plurality of reagent mixtures for hematology testing and analyzing particle distributions within the reagent mixtures for blood cell analysis, comprising:

means for pumping predetermined volumes of a plurality of reagent-mixture components, including at least a plurality of lysing agents, to create a plurality of reagent mixtures;

means for sensing particle distributions of the reagent mixtures for blood cell analysis; and

means for adjusting the reagent mixtures to correspond to each of a plurality of different operator inputs, said adjusting means selecting one or more of a plurality of lysing agents and controlling the pumping means in response to each input to adjust the predetermined volume of the at least one selected lysing agent to correspond to the respective input and thereby make a reagent mixture corresponding to the input, said adjusting means further controlling the pumping means to introduce the reagent mixture through the sensing means to sense a particle distribution of the reagent mixture for blood cell analysis.

9. An apparatus as defined in claim 8, wherein at least a plurality of the operator inputs each correspond to a respective species, and for each such input the plurality of reagent-mixture components includes a first reagent-mixture component consisting essentially of a whole blood sample of the respective species, a second reagent-mixture component consisting essentially of diluent, and a third reagent-mixture component consisting essentially of the at least one selected lysing agent for making a blood/diluent/lyse reagent mixture corresponding to the respective species.

10. An apparatus as defined in claim 8, wherein the means for adjusting the reagent mixture includes a database of predetermined quantities of each of a plurality of reagent-mixture components, and each predetermined quantity corresponds to a respective species, and wherein the means for adjusting the reagent mixture is responsive to each input corresponding to a respective species to control the pumping means to pump the predetermined quantity of each reagent-mixture component and make the reagent mixture for that species.

11. An apparatus for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures, comprising:

at least one pump;

a sensing unit defining a counting orifice for receiving a reagent mixture and analyzing a particle distribution of the reagent mixture; and

means for adjusting a reagent mixture including at least one lysing agent to correspond to each of a plurality of different operator inputs, said adjusting means selecting one or more lysing agents from a plurality of lysing agents, and controlling the at least one pump in response to each input to pump a predetermined volume of the at least one selected lysing agent and a predetermined volume of at least one other reagent-mixture component corresponding to the respective input, said adjusting means further controlling the at least one pump to intermix the predetermined quantities of the components and thereby create the reagent mixture corresponding to the respective input, and to introduce the reagent mixture through the counting orifice of the sensing unit for sensing a particle distribution of the reagent mixture.

12. An apparatus as defined in claim 11, wherein at least a plurality of the operator inputs each correspond to a respective species, and for each such input the plurality of reagent-mixture components includes a first reagent-mixture component consisting essentially of a whole blood sample of the respective species, a second reagent-mixture component consisting essentially of diluent, and a third reagent-mixture component consisting essentially of the at least one selected lysing agent for making a blood/diluent/lyse reagent mixture corresponding to the respective species.

13. An apparatus as defined in claim 11, wherein the means for adjusting the reagent mixture includes a database of predetermined quantities of each of a plurality of reagent-mixture components, and each predetermined quantity corresponds to a respective species, and wherein the means for adjusting the reagent mixture is responsive to each input to control the at least one pump to pump the predetermined quantity of each reagent-mixture component and make the reagent mixture for the respective species.

14. An apparatus as defined in claim 11, further comprising a mixing chamber coupled in fluid communication with the at least one pump and sensing unit for receiving and intermixing the predetermined quantities of the reagent-mixture components to thereby create the reagent mixture corresponding to the respective input prior to introduction of the reagent mixture through the counting orifice of the sensing unit.