Oxidative method for attachment of glycoproteins to surfaces of medical devices
First Claim
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1. A method of forming a coating on a surface of a medical device, the coating imparting biocompatibility characteristics to the surface, the method comprising the ordered steps of:
- (a) combining a periodate with a glycoprotein, the glycoprotein comprising a 1,2 dihydroxy moiety, to oxidize the 1,2 dihydroxy moiety to form an aldehyde-functional material;
(b) providing the medical device, the device having a suitable biomaterial forming the surface, an amine moiety being disposed on the surface;
(c) combining the aldehyde-functional material with the amine moiety to bond the aldehyde-functional material to the amine moiety and thereby form an imine moiety; and
(d) reacting the imine moiety with a reducing agent to form an amine linkage, the amine linkage immobilizing the glycoprotein on the surface, the immobilized glycoprotein forming the coating.
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Abstract
A method for making a medical device having a glycoprotein immobilized on a substrate surface is provided. The method includes oxidizing 1,2 dihydroxy moieties with a periodate to form an aldehyde-functional material; combining the aldehyde-functional material with an amino-functional material to bond the two materials together through an imine moiety; and reacting the imine moiety with a reducing agent to form a secondary amine.
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Citations
28 Claims
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1. A method of forming a coating on a surface of a medical device, the coating imparting biocompatibility characteristics to the surface, the method comprising the ordered steps of:
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(a) combining a periodate with a glycoprotein, the glycoprotein comprising a 1,2 dihydroxy moiety, to oxidize the 1,2 dihydroxy moiety to form an aldehyde-functional material; (b) providing the medical device, the device having a suitable biomaterial forming the surface, an amine moiety being disposed on the surface; (c) combining the aldehyde-functional material with the amine moiety to bond the aldehyde-functional material to the amine moiety and thereby form an imine moiety; and (d) reacting the imine moiety with a reducing agent to form an amine linkage, the amine linkage immobilizing the glycoprotein on the surface, the immobilized glycoprotein forming the coating. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 25)
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15. A method of crosslinking a coating on a surface of a medical device, the coating imparting biocompatibility characteristics to the surface, the method comprising the ordered steps of:
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(a) immobilizing a glycoprotein on the surface, the glycoprotein forming the coating and comprising a 1,2 dihydroxy moiety and an amine moiety; (b) applying a periodate to the coating to oxidize the 1,2 dihydroxy moiety to form an aldehyde moiety; (c) allowing the aldehyde moiety to combine with the amine moiety to form an imine moiety; and (d) reacting the imine moiety with a reducing agent to form a secondary amine and thereby cause at least portions of the coating to crosslink. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28)
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Specification