×

Oxidative method for attachment of glycoproteins to surfaces of medical devices

  • US 5,728,420 A
  • Filed: 08/09/1996
  • Issued: 03/17/1998
  • Est. Priority Date: 08/09/1996
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of forming a coating on a surface of a medical device, the coating imparting biocompatibility characteristics to the surface, the method comprising the ordered steps of:

  • (a) combining a periodate with a glycoprotein, the glycoprotein comprising a 1,2 dihydroxy moiety, to oxidize the 1,2 dihydroxy moiety to form an aldehyde-functional material;

    (b) providing the medical device, the device having a suitable biomaterial forming the surface, an amine moiety being disposed on the surface;

    (c) combining the aldehyde-functional material with the amine moiety to bond the aldehyde-functional material to the amine moiety and thereby form an imine moiety; and

    (d) reacting the imine moiety with a reducing agent to form an amine linkage, the amine linkage immobilizing the glycoprotein on the surface, the immobilized glycoprotein forming the coating.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×