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Process for categorizing nucleotide sequence populations

  • US 5,728,524 A
  • Filed: 01/13/1995
  • Issued: 03/17/1998
  • Est. Priority Date: 07/13/1992
  • Status: Expired due to Fees
First Claim
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1. A process for categorizing uncharacterized nucleic acid by sorting said nucleic acid into sequence-specific subsets comprising:

  • (a) optionally, cleaving said uncharacterized nucleic acid by the action of a reagent, which reagent cleaves said nucleic acid to produce smaller size cleavage products;

    (b) reacting either the uncharacterized nucleic acid or said cleavage products from (a) with a first population of adaptor molecules to generate first adaptored products, each of which first adaptor molecules comprises a nucleic acid sequence end recognition means, wherein said population of first adaptor molecules comprises a range of such molecules having sequence end recognition means linking, when present, to a first predetermined subset of said uncharacterized nucleic acid sequences to form first adaptored products;

    (c) selecting and separating said first adaptored products resulting from (b);

    (d) cleaving adaptors from the selected and separated first adaptored products from (c) to form first selected nucleic acids;

    (e) reacting said first selected nucleic acids resulting from (d) with a second population of adaptor molecules to generate second adaptored products, each of which second adaptor molecules comprises nucleic acid end recognition means, wherein said second population of adaptor molecules comprises sequence end recognition means linking to a second predetermined subset of nucleic acid sequences to form second adaptored products; and

    (f) selecting and separating said second adaptored products resulting from (e).

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