Vectors for use in filamentous fungi
First Claim
1. A DNA construct for use in transforming an Aspergillus host to obtain expression therein of a polypeptide, said DNA construct comprising inducible promoter DNA for promoting transcription in Aspergillus and operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus host, said DNA coding for said polypeptide being foreign to said promoter DNA.
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Reexamination
Accused Products
Abstract
Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.
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Citations
35 Claims
- 1. A DNA construct for use in transforming an Aspergillus host to obtain expression therein of a polypeptide, said DNA construct comprising inducible promoter DNA for promoting transcription in Aspergillus and operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus host, said DNA coding for said polypeptide being foreign to said promoter DNA.
- 13. An Aspergillus strain which upon culturing expresses a polypeptide as a result of having been transformed by a DNA construct in which DNA coding for the polypeptide is linked operably to inducible promoter DNA for promoting transcription in Aspergillus, said DNA coding for said polypeptide being foreign to said promoter DNA.
- 26. A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus strain transformed by a DNA construct in which DNA coding for a polypeptide is linked operably to inducible promoter DNA for promoting transcription in Aspergillus, said DNA coding for said polypeptide being foreign to said promoter DNA.
Specification